F3 / Tissue factor / CD142 Antibody

F3 / Tissue factor / CD142 Antibody Datasheet

F3 / Tissue factor / CD142 Antibody Product Information

• Immunogen: Recombinant Human F3 / Tissue factor / CD142 protein (Catalog#13133-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 05
• Ig Type: Mouse IgG2a
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human F3 / Tissue factor / CD142 (rh F3 / Tissue factor / CD142; Catalog#13133-H08H; P13726; Met 1-Glu 251). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

F3 / Tissue factor / CD142 Antibody Usage Guide

• Specificity: Human F3 / Tissue factor / CD142
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human F3. The detection limit for Human F3 is approximately 0.16 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

F3 / Tissue factor / CD142 Antibody Background

Tissue factor, also known as coagulation factor III, F3, and CD142, is a single-pass type I membrane protein which belongs to the tissue factor family. Tissue factor is one of the proteins that participate in hemostatic and inflammatory processes. Activated monocytes present in the liver increase expression of tissue factor, and while accumulating in the organ they can intensify inflammation. Tissue factor is the protein that activates the blood clotting system by binding to, and activating, the plasma serine protease, factor VIIa, following vascular injury. Because of its essential role in hemostasis, Tissue factor plays a role in pathology associated with hemostasis, triggering the coagulation system in many thrombotic diseases and the coagulopathies associated with sepsis and other forms of disseminated intravascular coagulation. Tissue factor is not only the main physiological initiator of normal blood coagulation, but is also important in the natural history of solid malignancies in that it potentiates metastasis and angiogenesis and mediates outside-in signalling. Tissue factor is expressed constitutively by many tissues which are not in contact with blood and by other cells upon injury or activation; the latter include endothelial cells, tissue macrophages, and peripheral blood monocytes.

References

1. Lwaleed BA. et al., 2001, J Pathol. 193 (1): 3-12.
2. Morrissey JH. 2004, Int J Hematol. 79 (2): 103-8.
3. Panasiuk A. et al., 2007, Blood Coagul Fibrinolysis. 18 (8): 739-44.
4. Kinev AV. et al., 2008, Lupus. 17 (10): 952-8.