After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!

Quick Order

Text Size:AAA

Coagulation Factor III / Tissue Factor / CD142 Antibody, Mouse MAb

DatasheetReviewsRelated ProductsProtocols
Human F3/CD142 Antibody Product Information
Immunogen:Recombinant Human F3 / Tissue factor / CD142 protein (Catalog#13133-H08H)
Clone ID:05
Ig Type:Mouse IgG2a
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human F3 / Tissue factor / CD142 (rh F3 / Tissue factor / CD142; Catalog#13133-H08H; P13726; Met 1-Glu 251). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human F3/CD142 Antibody Usage Guide
Specificity:Human F3 / Tissue factor / CD142
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)
Application:ELISA, ICC/IF, IF, FCM

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human F3. The detection limit for Human F3 is approximately 0.16 ng/well.

FCM: 0.5-2 μg/Test

ICC/IF: 10-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human F3/CD142 Antibody IHC Application Image
[Click to enlarge image]
Caption:
Immunofluorescence staining of Human F3 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human F3 monoclonal antibody (15 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue). Positive staining was localized to cell membrane and cytoplasm.
Coagulation Factor III / Tissue Factor / CD142 Antibody, Mouse MAb
Human F3/CD142 Antibody FC Application Image
[Click to enlarge image]
Caption:
Flow cytometric analysis of Human F3(CD142) expression on A431 cells. Cells were stained with purified anti-Human F3(CD142), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Coagulation Factor III / Tissue Factor / CD142 Antibody, Mouse MAb
Other F3/CD142 Antibody Products
Coagulation Factor III / Tissue Factor / CD142 Background

Tissue factor (TF), also known as coagulation factor III, F3, and CD142, is a single-pass type I membrane protein which belongs to the tissue factor family. Tissue factor is one of the proteins that participate in hemostatic and inflammatory processes. Activated monocytes present in the liver increase expression of tissue factor, and while accumulating in the organ they can intensify inflammation. Tissue factor is the protein that activates the blood clotting system by binding to, and activating, the plasma serine protease, factor VIIa, following vascular injury. Tissue factor is not only the main physiological initiator of normal blood coagulation, but is also important in the natural history of solid malignancies in that it potentiates metastasis and angiogenesis and mediates outside-in signalling. Tissue factor is expressed constitutively by many tissues which are not in contact with blood and by other cells upon injury or activation; the latter include endothelial cells, tissue macrophages, and peripheral blood monocytes. Coagulation Factor III is a transmembrane glycoprotein that localizes the coagulation serine protease factor VII/VIIa (FVII/VIIa) to the cell surface. The primary function of TF is to activate the clotting cascade. The TF:FVIIa complex also activates cells by cleavage of a G-protein coupled receptor called protease-activated receptor 2 (PAR2). TF is expressed by tumor cells and contributes to a variety of pathologic processes, such as thrombosis, metastasis, tumor growth, and tumor angiogenesis. As a key regulator of haemostasis and angiogenesis, it is also involved in the pathology of several diseases, including cardiovascular, inflammatory and neoplastic conditions.

Human Coagulation Factor III / Tissue Factor / CD142 References
  • Morrissey JH. (2004) Tissue factor: a key molecule in hemostatic and nonhemostatic systems. Int J Hematol. 79(2): 103-8.
  • Milsom C, et al. (2008) Tissue factor and cancer. Pathophysiol Haemost Thromb. 36(3-4): 160-76.
  • Kasthuri RS, et al. (2009) Role of tissue factor in cancer. J Clin Oncol. 27(29): 4834-8.
  • Product nameProduct name
    Size / Price
    Catalog: 13133-MM05-50
    List Price:   (Save )
    Price:      [How to order]
    AvailabilityIn Stock
     Shipping instructions
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"