Human F2RL1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13536-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

F2RL1 cDNA Clone Datasheet

F2RL1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_005242.4
cDNA Size:	1194
Gene Synonym:	PAR2; GPR11；F2RL1
Source:	Human

F2RL1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens coagulation factor II (thrombin) receptor-like 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 1192-1194 TGA/TAA not causing the amino acid variation.

F2RL1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

F2RL1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

F2RL1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGGAGCC CCAGCGCGGC GTGGCTGCTG GGGGCCGCCA TCCTGCTAGC AGCCTCTCTC
61       TCCTGCAGTG GCACCATCCA AGGAACCAAT AGATCCTCTA AAGGAAGAAG CCTTATTGGT
121      AAGGTTGATG GCACATCCCA CGTCACTGGA AAAGGAGTTA CAGTTGAAAC AGTCTTTTCT
181      GTGGATGAGT TTTCTGCATC TGTCCTCACT GGAAAACTGA CCACTGTCTT CCTTCCAATT
241      GTCTACACAA TTGTGTTTGT GGTGGGTTTG CCAAGTAACG GCATGGCCCT GTGGGTCTTT
301      CTTTTCCGAA CTAAGAAGAA GCACCCTGCT GTGATTTACA TGGCCAATCT GGCCTTGGCT
361      GACCTCCTCT CTGTCATCTG GTTCCCCTTG AAGATTGCCT ATCACATACA TGGCAACAAC
421      TGGATTTATG GGGAAGCTCT TTGTAATGTG CTTATTGGCT TTTTCTATGG CAACATGTAC
481      TGTTCCATTC TCTTCATGAC CTGCCTCAGT GTGCAGAGGT ATTGGGTCAT CGTGAACCCC
541      ATGGGGCACT CCAGGAAGAA GGCAAACATT GCCATTGGCA TCTCCCTGGC AATATGGCTG
601      CTGATTCTGC TGGTCACCAT CCCTTTGTAT GTCGTGAAGC AGACCATCTT CATTCCTGCC
661      CTGAACATCA CGACCTGTCA TGATGTTTTG CCTGAGCAGC TCTTGGTGGG AGACATGTTC
721      AATTACTTCC TCTCTCTGGC CATTGGGGTC TTTCTGTTCC CAGCCTTCCT CACAGCCTCT
781      GCCTATGTGC TGATGATCAG AATGCTGCGA TCTTCTGCCA TGGATGAAAA CTCAGAGAAG
841      AAAAGGAAGA GGGCCATCAA ACTCATTGTC ACTGTCCTGG CCATGTACCT GATCTGCTTC
901      ACTCCTAGTA ACCTTCTGCT TGTGGTGCAT TATTTTCTGA TTAAGAGCCA GGGCCAGAGC
961      CATGTCTATG CCCTGTACAT TGTAGCCCTC TGCCTCTCTA CCCTTAACAG CTGCATCGAC
1021     CCCTTTGTCT ATTACTTTGT TTCACATGAT TTCAGGGATC ATGCAAAGAA CGCTCTCCTT
1081     TGCCGAAGTG TCCGCACTGT AAAGCAGATG CAAGTATCCC TCACCTCAAA GAAACACTCC
1141     AGGAAATCCA GCTCTTACTC TTCAAGTTCA ACCACTGTTA AGACCTCCTA TTAA

Quality Control:cDNA sequencing results is available upon request.