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Human F2 / FII Gene cDNA Clone (full-length ORF Clone)
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Human F2 / FII Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG11505-M





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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F2/FII cDNA Clone Datasheet

 

F2/FII cDNA Clone Product Information

Gene Bank Ref.ID: NM_000506.3

cDNA Size:

1869

Gene Synonym:

PT; F2

Source: Human

F2/FII cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens coagulation factor II (thrombin) DNA.

Vector: Cloning Vector: pMD18-T Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

F2/FII cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

F2/FII cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

F2/FII cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGCACG TCCGAGGCTT GCAGCTGCCT GGCTGCCTGG CCCTGGCTGC CCTGTGTAGC
61       CTTGTGCACA GCCAGCATGT GTTCCTGGCT CCTCAGCAAG CACGGTCGCT GCTCCAGCGG
121      GTCCGGCGAG CCAACACCTT CTTGGAGGAG GTGCGCAAGG GCAACCTGGA GCGAGAGTGC
181      GTGGAGGAGA CGTGCAGCTA CGAGGAGGCC TTCGAGGCTC TGGAGTCCTC CACGGCTACG
241      GATGTGTTCT GGGCCAAGTA CACAGCTTGT GAGACAGCGA GGACGCCTCG AGATAAGCTT
301      GCTGCATGTC TGGAAGGTAA CTGTGCTGAG GGTCTGGGTA CGAACTACCG AGGGCATGTG
361      AACATCACCC GGTCAGGCAT TGAGTGCCAG CTATGGAGGA GTCGCTACCC ACATAAGCCT
421      GAAATCAACT CCACTACCCA TCCTGGGGCC GACCTACAGG AGAATTTCTG CCGCAACCCC
481      GACAGCAGCA CCACGGGACC CTGGTGCTAC ACTACAGACC CCACCGTGAG GAGGCAGGAA
541      TGCAGCATCC CTGTCTGTGG CCAGGATCAA GTCACTGTAG CGATGACTCC ACGCTCCGAA
601      GGCTCCAGTG TGAATCTGTC ACCTCCATTG GAGCAGTGTG TCCCTGATCG GGGGCAGCAG
661      TACCAGGGGC GCCTGGCGGT GACCACACAT GGGCTCCCCT GCCTGGCCTG GGCCAGCGCA
721      CAGGCCAAGG CCCTGAGCAA GCACCAGGAC TTCAACTCAG CTGTGCAGCT GGTGGAGAAC
781      TTCTGCCGCA ACCCAGACGG GGATGAGGAG GGCGTGTGGT GCTATGTGGC CGGGAAGCCT
841      GGCGACTTTG GGTACTGCGA CCTCAACTAT TGTGAGGAGG CCGTGGAGGA GGAGACAGGA
901      GATGGGCTGG ATGAGGACTC AGACAGGGCC ATCGAAGGGC GTACCGCCAC CAGTGAGTAC
961      CAGACTTTCT TCAATCCGAG GACCTTTGGC TCGGGAGAGG CAGACTGTGG GCTGCGACCT
1021     CTGTTCGAGA AGAAGTCGCT GGAGGACAAA ACCGAAAGAG AGCTCCTGGA ATCCTACATC
1081     GACGGGCGCA TTGTGGAGGG CTCGGATGCA GAGATCGGCA TGTCACCTTG GCAGGTGATG
1141     CTTTTCCGGA AGAGTCCCCA GGAGCTGCTG TGTGGGGCCA GCCTCATCAG TGACCGCTGG
1201     GTCCTCACCG CCGCCCACTG CCTCCTGTAC CCGCCCTGGG ACAAGAACTT CACCGAGAAT
1261     GACCTTCTGG TGCGCATTGG CAAGCACTCC CGCACCAGGT ACGAGCGAAA CATTGAAAAG
1321     ATATCCATGT TGGAAAAGAT CTACATCCAC CCCAGGTACA ACTGGCGGGA GAACCTGGAC
1381     CGGGACATTG CCCTGATGAA GCTGAAGAAG CCTGTTGCCT TCAGTGACTA CATTCACCCT
1441     GTGTGTCTGC CCGACAGGGA GACGGCAGCC AGCTTGCTCC AGGCTGGATA CAAGGGGCGG
1501     GTGACAGGCT GGGGCAACCT GAAGGAGACG TGGACAGCCA ACGTTGGTAA GGGGCAGCCC
1561     AGTGTCCTGC AGGTGGTGAA CCTGCCCATT GTGGAGCGGC CGGTCTGCAA GGACTCCACC
1621     CGGATCCGCA TCACTGACAA CATGTTCTGT GCTGGTTACA AGCCTGATGA AGGGAAACGA
1681     GGGGATGCCT GTGAAGGTGA CAGTGGGGGA CCCTTTGTCA TGAAGAGCCC CTTTAACAAC
1741     CGCTGGTATC AAATGGGCAT CGTCTCATGG GGTGAAGGCT GTGACCGGGA TGGGAAATAT
1801     GGCTTCTACA CACATGTGTT CCGCCTGAAG AAGTGGATAC AGAAGGTCAT TGATCAGTTT
1861     GGAGAGTAG

 

Quality Control:cDNA sequencing results is available upon request.


 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"