Human F12 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12281-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

F12 cDNA Clone Datasheet

F12 cDNA Clone Product Information

Gene Bank Ref.ID:	BC012390
cDNA Size:	903
Gene Synonym:	HAF; HAE3; HAEX; F12
Source:	Human

F12 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens coagulation factor XII (Hageman factor) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

F12 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

F12 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

F12 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCCGCGC AGCCGGCACC GCCGAAGCCT CAGCCCACGA CCCGGACCCC GCCTCAGTCC
61       CAGACCCCGG GAGCCTTGCC GGCGAAGCGG GAGCAGCCGC CTTCCCTGAC CAGGAACGGC
121      CCACTGAGCT GCGGGCAGCG GCTCCGCAAG AGTCTGTCTT CGATGACCCG CGTCGTTGGC
181      GGGCTGGTGG CGCTACGCGG GGCGCACCCC TACATCGCCG CGCTGTACTG GGGCCACAGT
241      TTCTGCGCCG GCAGCCTCAT CGCCCCCTGC TGGGTGCTGA CGGCCGCTCA CTGCCTGCAG
301      GACCGGCCCG CACCCGAGGA TCTGACGGTG GTGCTCGGCC AGGAACGCCG TAACCACAGC
361      TGTGAGCCGT GCCAGACGTT GGCCGTGCGC TCCTACCGCT TGCACGAGGC CTTCTCGCCC
421      GTCAGCTACC AGCACGACCT GGCTCTGTTG CGCCTTCAGG AGGATGCGGA CGGCAGCTGC
481      GCGCTCCTGT CGCCTTACGT TCAGCCGGTG TGCCTGCCAA GCGGCGCCGC GCGACCCTCC
541      GAGACCACGC TCTGCCAGGT GGCCGGCTGG GGCCACCAGT TCGAGGGGGC GGAGGAATAT
601      GCCAGCTTCC TGCAGGAGGC GCAGGTACCG TTCCTCTCCC TGGAGCGCTG CTCAGCCCCG
661      GACGTGCACG GATCCTCCAT CCTCCCCGGC ATGCTCTGCG CAGGGTTCCT CGAGGGCGGC
721      ACCGATGCGT GCCAGGGTGA TTCCGGAGGC CCGCTGGTGT GTGAGGACCA AGCTGCAGAG
781      CGCCGGCTCA CCCTGCAAGG CATCATCAGC TGGGGATCGG GCTGTGGTGA CCGCAACAAG
841      CCAGGCGTCT ACACCGATGT GGCCTACTAC CTGGCCTGGA TCCGGGAGCA CACCGTTTCC
901      TGA

Quality Control:cDNA sequencing results is available upon request.