Rat F11R Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80240-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

F11R cDNA Clone Datasheet

F11R cDNA Clone Product Information

Gene Bank Ref.ID:	NM_053796.1
cDNA Size:	903
Gene Synonym:	Jam1; F11r
Source:	Rat

F11R cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus F11 receptor DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 132 T/C not causing the amino acid variation.

F11R cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

F11R cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

F11R cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCACCG AGGGGAAAGC CGGGAGCAAA CTGTTGTTTC TCTTCACGTC TATGATCCTG
61       GGCTCCTTGG TGCAAGGCAA GGGTTCGGTG TACAGTCCTC AGACTGCCGT CCAGGTTCCT
121      GAGAACGACT CCGTCAAGTT GCCCTGTATC TACTCTGGCT TCTCCTCACC CCGCGTGGAG
181      TGGAAGTTCG TCCAAGGCAG CACCACTGCG CTTGTGTGCT ATAACAACCA GATCACAGTT
241      CCCTATGCAG ACCGCGTCAC CTTCTCATCC AGTGGCATCA CCTTCAGCTC TGTGACCCGG
301      AAAGACAATG GAGAGTATAC TTGCATGGTC TCTGAGGACG GGGGTCAGAA CTACGGGGAG
361      GTCAGCATCC ACCTCACTGT GCTTGTGCCT CCATCCAAGC CGACAGTCAG TATCCCCTCC
421      TCTGTCACCA TTGGGAACAG GGCAGTGCTG ACCTGCTCAG AACACGACGG TTCCCCTCCT
481      TCTGAATATT CCTGGTTCAA GGATGGGGTA CCTATGCTTA CAGCAGATGC CAAGAAAACC
541      CGTGCCTTCA TCAATTCTTC ATACACCATC GATCCAAAGT CAGGGGATTT GGTCTTTGAC
601      CCTGTGTCAG CCTTTGATAG TGGCGAATAC TACTGCGAGG CACAGAACGG GTATGGGACA
661      GCCATGAGGT CAGAGGCTGT ACGCATGGAG GCTGTGGAGC TGAATGTGGG GGGCATCGTG
721      GCAGCTGTCC TGGTAACACT GATTCTCCTT GGACTCCTGA TTTTTGGCAT CTGGTTTGCC
781      TATAGCCGTG GATACTTTGA AAGAACAAAG AAAGGGACTG CACCGGGTAA GAAGGTCATT
841      TACAGCCAGC CCAGTGCTCG CAGTGAGGGA GAATTCAAAC AGACCTCGTC ATTCCTGGTG
901      TGA

Quality Control:cDNA sequencing results is available upon request.