Rat F11R / JAM-A / JAM-1 Protein (His Tag)

F11 receptor Protein Datasheet

F11R / JAM-A / JAM-1 Protein Product Information

• Synonym: F11R, Jam1
• Protein Construction: A DNA sequence encoding the rat F11R (Q9JHY1)(Met1-Gly238) was expressed with a polyhistidine tag at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

F11R / JAM-A / JAM-1 Protein QC Testing

• Purity: > (23.7+75.2) % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Lys 27
• Molecular Mass: The recombinant rat F11Rcomprises 223 amino acids and predicts a molecular mass of 24.3 kDa. The apparent molecular mass of the recombinant protein is approximately 28 and 32 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: F11R / JAM-A / JAM-1 protein

F11R / JAM-A / JAM-1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

F11R / JAM-A / JAM-1 Protein Description

Junctional adhesion molecule A, also known as Junctional adhesion molecule 1, Platelet F11 receptor, JAM-1, Platelet adhesion molecule 1, JAM-A, PAM-1, CD321 and F11R, is a single-pass type I  membrane protein which belongs to the immunoglobulin superfamily and Junctional Adhesion Molecule (JAM) family. JAM-A / CD321 is the firstly identified member of the JAM family comprising at least three members. JAM-A is specifically localized in tight junctions of epithelial and endothelial cells and is involved in the regulation of junctional integrity and permeability by serving as a physical barrier in a homophilic manner. JAM-A is also expressed on the surface of hematopoeitic cells, such as platelets and leukocytes, and acts as a ligand for integrin LFA-1 or a platelet receptor, consequently plays key roles in a variety of cellular processes, including platelet aggregation, leukocyte transmigration, and angiogenesis. In addition, JAM-A has also been implicated in the attachment of reovirus that causes diarrhea.

References

1. Williams LA. et al., 1999, Mol Immunol. 36: 1175-88.
2. Sobocka MB. et al., 2000, Blood. 95: 2600-9.
3. Babinska A. et al., 2002, Thromb Haemost. 88: 843-50.
4. Ostermann G. et al., 2002, Nat Immunol. 3: 151-8.
5. Kenneth J. et al., 2005, J Biol Chem. 280: 11665-74.
6. Bazzoni G. et al., 2003, Curr Opin Cell Biol. 15: 525-30.
7. Barton ES. et al., 2001, Cell. 104 : 441-51.