Escherichia coli acnB Antibody (Antigen Affinity Purified)

Escherichia coli acnB Antibody ( Antigen Affinity Purified ) Datasheet

Escherichia coli acnB Antibody Product Information

• Immunogen: Recombinant Escherichia coli acnB protein (Catalog#13102-ENAE)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Escherichia coli acnB (Catalog#13102-ENAE; Met 1-Val 865). Escherichia coli acnB specific IgG was purified by Escherichia coli acnB affinity chromatography.

Escherichia coli acnB Antibody Usage Guide

• Specificity: Escherichia coli acnB
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect E. coli AcnB in WB. Using a DAB detection system, the detection limit for E. coli AcnB is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect E. coli AcnB. The detection limit for E. coli AcnB is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Escherichia coli acnB Antibody Background

Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains. acnA is specifically subject to SoxRS-mediated activation, whereas acnB encodes the major aconitase that is synthesized earlier in the growth cycle than AcnA. It is concluded that AcnB is the major citric acid cycle enzyme. Aconitate hydratase 2 (acnB) catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate, thus it functions as the major citric-acid-cycle enzyme during exponential growth. Escherichia coli acnB serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster. AcnB represents a large, distinct group of Gram-negative bacterial aconitases that have an altered domain organization relative to mitochondrial aconitase and other aconitases.

References

1. Bradbury AJ. et al., 1996, 142 (Pt 2): 389-400.
2. Cunningham L. et al., 1997, Microbiology. 143 (Pt 12): 3795-805.
3. Jordan PA. et al., 1999, Biochem J. 344 Pt 3: 739-46.
4. Williams CH. et al., 2002, Nat Struct Biol. 9 (6): 447-52.