Anti-acnB Antibody

Cat: 13102-RP01
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Anti-acnB Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-acnB Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: E. coli
Specificity
E. coli acnB
Immunogen
Recombinant Escherichia coli acnB protein (Catalog#13102-ENAE)
Preparation
Produced in rabbits immunized with purified, recombinant Escherichia coli acnB (Catalog#13102-ENAE; Met 1-Val 865). Total IgG was purified by Protein A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-acnB Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect E. coli AcnB in WB.  
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-acnB Antibody Alternative Names
Anti-yacI Antibody;Anti-yacJ Antibody
acnB Background Information

Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains. acnA is specifically subject to SoxRS-mediated activation, whereas acnB encodes the major aconitase that is synthesized earlier in the growth cycle than AcnA. It is concluded that AcnB is the major citric acid cycle enzyme. Aconitate hydratase 2 (acnB) catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate, thus it functions as the major citric-acid-cycle enzyme during exponential growth. Escherichia coli acnB serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster. AcnB represents a large, distinct group of Gram-negative bacterial aconitases that have an altered domain organization relative to mitochondrial aconitase and other aconitases.

References
  • Bradbury AJ. et al., 1996, 142 (Pt 2): 389-400.
  • Cunningham L. et al., 1997, Microbiology. 143 (Pt 12): 3795-805.
  • Jordan PA. et al., 1999, Biochem J. 344 Pt 3: 739-46.
  • Williams CH. et al., 2002, Nat Struct Biol. 9(6): 447-52.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-acnB Antibody Mouse Monoclonal ELISA(Det) 13102-MM02
    Anti-acnB Antibody Mouse Monoclonal ELISA(Cap) 13102-MM09
    Anti-acnB Antibody Mouse Monoclonal WB,ELISA 13102-MM05
    Anti-acnB Antibody Rabbit Monoclonal ELISA 13102-R102
    Anti-acnB Antibody Rabbit Polyclonal ELISA 13102-RP02
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