Human / Rhesus ErbB4 / HER4 Protein (His Tag)

V-erb-a Erythroblastic Leukemia Viral Oncogene Homolog 4 Protein Datasheet

ErbB4 / HER4 Protein Product Information

• Synonym: ERBB4, HER4
• Protein Construction: A DNA sequence encoding the human ERBB4(NP_005226.1) (Met1-Arg649 )with a C-terminal polyhistidine tag was expressed. Human and Rhesus ERBB4 sequences are identical.
• Source: Human
• Expression Host: Human Cells

ErbB4 / HER4 Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 26
• Molecular Mass: The recombinant human ERBB4 is a disulfide-linked homodimer. The reduced monomer comprises 635 amino acids and has a predicted molecular mass of 71.1 kDa. The apparent molecular mass of the protein is approximately 102 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ErbB4 / HER4 protein

ErbB4 / HER4 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ErbB4 / HER4 Protein Description

ErbB4, also known as Her4, is a member of the tyrosine kinase receptors (RTK) and the epidermal growth factor receptor (EGFR) subfamily, and appears to play important roles in differentiation, development and certain carcinomas. It is a single transmembrane-span type I  glycoprotein structurally consisting of multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 (PI-3) kinase binding site and a PDZ domain binding motif. However, the last two domains are absent in one of the two naturally occurring ErbB4 isoforms, ErbB4 CYT-2. ErbB4 ligands include the neuregulins, betacellulin and heparin-binding EGF-like growth factor (HB-EGF). Typically, heterodimerization of ErbB4 with ErbB2 forms the high affinity receptor complex, and subsequnetly transmit cellular signals by activating downstream src homology-2 (SH2) and phosphotyrosine binding (PTB) domain-containing signaling molecules.. ErbB4 undergoes sequential processing by TNF-α converting enzyme (TACE) and presenilin-dependent gamma-secretase to release the ErbB4 ICD (4ICD) with the NLS sequence which is essential for nuclear accumulation of 4ICD. It has been shown that the ligand-indued proteolytic process of ErbB4 results in the accumulation of 4ICD and cytochrome c efflux, and directly couples a cell surface signal to the tumor cell mitochondrial apoptotic pathway. Accordingly, in contrast to the other three members of the EGFR family, ErbB4 expression seems to be selectively lost in tumors with aggressive phenotypes.

References

1. Elenius K. et al., 1997, EMBO J. 16: 1268-78.
2. Vidal GA. et al., 2005, J Biol Chem. 280: 19777-83.
3. Carpenter G. 2003, Exp Cell Res. 284: 66-77.
4. Vidal GA. et al., 2007, Oncogene. 26: 462-6.
5. Kainulainen V. et al., 2000, J Biol Chem. 275: 8641-9.
6. Naresh A. et al., 2006, Cancer Res. 66: 6412-20.