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>Human Cell Expressed
>Rat HER4 / ErbB4 Protein (Fc Tag)
|Catalog||Size (Price)||Quantity||In Stock||Operation|
v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) Protein Datasheet
ErbB4 / HER4 Protein Price Inquiry ( Available Sizes )
ErbB4 / HER4 Protein Product Information
|Synonym :||ERBB4, Tyro-2|
A DNA sequence encoding the rat ERBB4 (Met1-Pro651) was expressed with the Fc region of human IgG1 at the C-terminus.
|Expression Host:||Human Cells|
ErbB4 / HER4 Protein QC Testing
|Purity:||> 95% as determined by SDS-PAGE||SDS-PAGE:
ErbB4 / HER4 protein
|Measured by its binding ability in a functional ELISA.
Immobilized rat ERBB4-Fc at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (Cat:11609-H01H2), The EC50 of biotinylated human NRG1 (Cat:11609-H01H2) is 0.69-1.61 μg/ml.
|Endotoxin:||< 1.0 EU per μg of the protein as determined by the LAL method|
|Stability:||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal:||Gln 26|
The recombinant rat ERBB4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 867 amino acids and has a predicted molecular mass of 96.9 kDa. The apparent molecular mass of the protein is approximately 119 kDa in SDS-PAGE under reducing conditions.
|Formulation:||Lyophilized from sterile PBS, pH7.4.
ErbB4 / HER4 Protein Usage Guide
|Storage:||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution:||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
ErbB4 / HER4 Protein Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|ErbB4/HER4||Human||ErbB4 / HER4 Protein, Recombinant||10363-H02H|
|ErbB4/HER4||Human||ErbB4/HER4/Fc Protein, Recombinant||10363-H03H|
|ErbB4/HER4||Human||ErbB4 / HER4 Protein, Recombinant||10363-H08H|
|ErbB4/HER4||Mouse||ErbB4 / HER4 Protein, Recombinant||51064-M08H|
|ErbB4/HER4||Rat||ErbB4 / HER4 Protein, Recombinant||80125-R02H|
ErbB4 / HER4 Protein Description
ErbB4, also known as Her4, is a member of the tyrosine kinase receptors (RTK) and the epidermal growth factor receptor (EGFR) subfamily, and appears to play important roles in differentiation, development and certain carcinomas. It is a single transmembrane-span type I glycoprotein structurally consisting of multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 (PI-3) kinase binding site and a PDZ domain binding motif. However, the last two domains are absent in one of the two naturally occurring ErbB4 isoforms, ErbB4 CYT-2. ErbB4 ligands include the neuregulins, betacellulin and heparin-binding EGF-like growth factor (HB-EGF). Typically, heterodimerization of ErbB4 with ErbB2 forms the high affinity receptor complex, and subsequnetly transmit cellular signals by activating downstream src homology-2 (SH2) and phosphotyrosine binding (PTB) domain-containing signaling molecules.. ErbB4 undergoes sequential processing by TNF-α converting enzyme (TACE) and presenilin-dependent gamma-secretase to release the ErbB4 ICD (4ICD) with the NLS sequence which is essential for nuclear accumulation of 4ICD. It has been shown that the ligand-indued proteolytic process of ErbB4 results in the accumulation of 4ICD and cytochrome c efflux, and directly couples a cell surface signal to the tumor cell mitochondrial apoptotic pathway. Accordingly, in contrast to the other three members of the EGFR family, ErbB4 expression seems to be selectively lost in tumors with aggressive phenotypes.
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