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Rat HER4 / ErbB4 Protein (Fc Tag)
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Rat HER4 / ErbB4 Protein (Fc Tag) PDF Download

Catalog   Size (Price) Quantity In Stock Operation
80125-R02H  
YES   
 

Protein Production & Purification Service | Recombinant Cytokines & Growth Factors

v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) Protein Datasheet

 

 

ErbB4 / HER4 Protein Price Inquiry ( Available Sizes )

ErbB4 / HER4 Protein Product Information

Synonym : ERBB4, Tyro-2
Protein Construction:

A DNA sequence encoding the rat ERBB4 (Met1-Pro651) was expressed with the Fc region of human IgG1 at the C-terminus.

Source: Rat
Expression Host: Human Cells

ErbB4 / HER4 Protein QC Testing

Purity: > 95% as determined by SDS-PAGE SDS-PAGE:
SDS-PAGE

ErbB4 / HER4 protein

Bio-activity:

Measured by its binding ability in a functional ELISA.
Immobilized rat ERBB4-Fc at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (Cat:11609-H01H2), The EC50 of biotinylated human NRG1 (Cat:11609-H01H2) is 0.69-1.61 μg/ml.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
Stability: Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal: Gln 26
Molecular Mass:

The recombinant rat ERBB4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 867 amino acids and has a predicted molecular mass of 96.9 kDa. The apparent molecular mass of the protein is approximately 119 kDa in SDS-PAGE under reducing conditions.

Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

ErbB4 / HER4 Protein Usage Guide

Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ErbB4 / HER4 Protein Related Products & Topics

Related Areas:

Enzyme>>Protein Kinase>>Receptor Tyrosine Kinase>>ErbB4/HER4

Cancer>>Cancer Biomarkers>>ErbB4/HER4

Cancer>>Growth Factor & Receptor>>EGF Family & Receptor>>ErbB4/HER4

Cancer>>Growth Factor & Receptor>>Receptor Tyrosine Kinase>>ErbB4/HER4

Proteins:

Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
ErbB4/HER4 Human ErbB4 / HER4 Protein, Recombinant 10363-H02H
ErbB4/HER4 Human ErbB4/HER4/Fc Protein, Recombinant 10363-H03H
ErbB4/HER4 Human ErbB4 / HER4 Protein, Recombinant 10363-H08H
ErbB4/HER4 Mouse ErbB4 / HER4 Protein, Recombinant 51064-M08H
ErbB4/HER4 Rat ErbB4 / HER4 Protein, Recombinant 80125-R02H

ErbB4 / HER4 Protein Description

ErbB4, also known as Her4, is a member of the tyrosine kinase receptors (RTK) and the epidermal growth factor receptor (EGFR) subfamily, and appears to play important roles in differentiation, development and certain carcinomas. It is a single transmembrane-span type I  glycoprotein structurally consisting of multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 (PI-3) kinase binding site and a PDZ domain binding motif. However, the last two domains are absent in one of the two naturally occurring ErbB4 isoforms, ErbB4 CYT-2. ErbB4 ligands include the neuregulins, betacellulin and heparin-binding EGF-like growth factor (HB-EGF). Typically, heterodimerization of ErbB4 with ErbB2 forms the high affinity receptor complex, and subsequnetly transmit cellular signals by activating downstream src homology-2 (SH2) and phosphotyrosine binding (PTB) domain-containing signaling molecules.. ErbB4 undergoes sequential processing by TNF-α converting enzyme (TACE) and presenilin-dependent gamma-secretase to release the ErbB4 ICD (4ICD) with the NLS sequence which is essential for nuclear accumulation of 4ICD. It has been shown that the ligand-indued proteolytic process of ErbB4 results in the accumulation of 4ICD and cytochrome c efflux, and directly couples a cell surface signal to the tumor cell mitochondrial apoptotic pathway. Accordingly, in contrast to the other three members of the EGFR family, ErbB4 expression seems to be selectively lost in tumors with aggressive phenotypes.

References

  1. Elenius K. et al., 1997, EMBO J. 16: 1268-78.
  2. Vidal GA. et al., 2005, J Biol Chem. 280: 19777-83.
  3. Carpenter G. 2003, Exp Cell Res. 284: 66-77.
  4. Vidal GA. et al., 2007, Oncogene. 26: 462-6.
  5. Kainulainen V. et al., 2000, J Biol Chem. 275: 8641-9.
  6. Naresh A. et al., 2006, Cancer Res. 66: 6412-20.
 

Related Information

EGFR (ErbB1, HER1)

ErbB2 (HER2)

ErbB3 (HER3)

ErbB4 (HER4)

 

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"