ErbB3 / HER3 (aa 730-1065) Protein (GST Tag)

V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 Protein Datasheet

ErbB3 / HER3 Protein Product Information

• Synonym: HER3，LCCS2，ErbB-3，c-erbB3，erbB3-S，MDA-BF-1，MGC88033，c-erbB-3， p180-ErbB3，p45-sErbB3，p85-sErbB3
• Protein Construction: A DNA sequence encoding the cytoplasmic domain (Pro 730-Ser 1065) of human ErbB3 (NP_001973.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

ErbB3 / HER3 Protein QC Testing

• Purity: > 82 % as determined by SDS-PAGE
• Bio-activity: Using the Octet RED System, the affinity constant (Kd) of human ErBB3-GST (Cat:10201-H20B1) bound to human NRG1 (aa 2-224) (Cat:11609-H04H) was 20 nM.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human ErbB3 /GST chimera consists of 573 amino acids and has a calculated molecular mass of 65 KDa. It migrates as an approximately 65 KDa band as predicted in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 50mM Tris,100mM NaCl, pH 7.5, 10%gly, 1mM GSH.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ErbB3 / HER3 protein

ErbB3 / HER3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ErbB3 / HER3 Protein Description

ErbB3, also known as Her3 (human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

References

1. Kraus MH. et al., 1989, Proc Natl Acad Sci. 86: 9193-7.
2. Carraway K.L. et al., 1994, J Biol Chem. 269: 14303 -6.
3. Wallasch C. et al., 1995, EMBO. J. 14: 4267-75.
4. Alimandi M. et al., 1995, Oncogene. 10:1813-21.
5. Gamett DC. et al., 1995, J Biol Chem. 270: 19022-7.
6. Katoh M. et al., 1993, Biochem Biophys Res Commun.192: 1189-97.