Rhesus ErbB3 / HER3 Protein (His Tag)

V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) Protein Datasheet

ErbB3 / HER3 Protein Product Information

• Synonym: ErbB3
• Protein Construction: A DNA sequence encoding the Rhesus （Macaca mulatta）ErbB3 (XP_001113953.2) extracellular domain (Met 1-Thr 643) was fused with a polyhistidine tag at the C-terminus.
• Source: Rhesus
• Expression Host: Human Cells

ErbB3 / HER3 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized Rhesus ErbB3 at 2 μg/mL (100 μl/well) can bind human NRG1 (isoform Beta1), The EC₅₀ of human NRG1 (isoform Beta1) is 0.58 μg/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 20
• Molecular Mass: The recombinant Rhesus ErbB3 consists of 635 amino acids and predicts a molecular mass of 70.1 KDa. As a result of glycosylation, rhesus ErbB3 migrates as an approximately 100 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ErbB3 / HER3 protein

ErbB3 / HER3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ErbB3 / HER3 Protein Description

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

References

1. Kraus,M.H. et al., 1989, Proc. Natl. Acad. Sci. 86: 9193-9197.
2. Carraway, K.L. et al., 1994, J. Biol. Chem. 269: 14303 -14306.
3. Wallasch, C. et al., 1995, EMBO. J. 14: 4267-4275.
4. Alimandi, M. et al., 1995, Oncogene. 10:1813-1821.
5. Gamett, D.C. et al., 1995, J. Biol. Chem. 270 : 19022-19027.
6. Katoh, M. et al., 1993, Biochem. Biophys. Res.Commun. 192: 1189-1197