ErbB3 / HER3 Antibody

ErbB3 / HER3 Antibody Datasheet

ErbB3 / HER3 Antibody Product Information

• Immunogen: Recombinant Rhesus ErbB3 / HER3 protein (Catalog#90043-C08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 05
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rhesus ErbB3 / HER3 (Catalog#90043-K08H; XP_001113953.2; Met 1-Thr 643). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

ErbB3 / HER3 Antibody Usage Guide

• Specificity: Rhesus ErbB3 / HER3
• Has cross-reactivity in ELISA with
  

  Human ERBB3

  Mouse ERBB3

  No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Rhesus ErbB3. The detection limit for Rhesus ErbB3 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

ErbB3 / HER3 Antibody Background

ErbB3, also known as Her3 (human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

References

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