Mouse EFNB2 Gene cDNA Clone / ORF Clone

EFNB2 cDNA Clone Datasheet

EFNB2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010111.5
• cDNA Size: 1011
• Gene Synonym: Epl5; ELF-2; Eplg5; Htk-L; Lerk5; LERK-5; NLERK-1; Efnb2
• Source: Mouse

EFNB2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Ephrin-B2 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

EFNB2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EFNB2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

EFNB2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCATGG CCCGGTCCAG GAGGGACTCT GTGTGGAAGT ACTGTTGGGG ACTTTTGATG
61       GTTTTGTGCA GAACTGCGAT CTCCAGATCG ATAGTTTTAG AGCCTATCTA CTGGAATTCC
121      TCGAACTCCA AATTTCTACC CGGACAAGGC CTGGTACTAT ACCCACAGAT AGGAGACAAA
181      TTGGATATTA TTTGCCCCAA AGTGGACTCT AAAACTGTTG GCCAGTATGA ATATTATAAA
241      GTTTATATGG TTGATAAAGA CCAAGCAGAC AGATGCACAA TTAAGAAGGA GAATACCCCG
301      CTGCTCAACT GTGCCAGACC AGACCAAGAT GTGAAATTCA CCATCAAGTT TCAAGAATTC
361      AGCCCTAACC TCTGGGGTCT AGAATTTCAG AAGAACAAAG ATTACTACAT TATATCTACA
421      TCAAATGGGT CTTTGGAGGG CCTGGATAAC CAGGAGGGAG GGGTGTGCCA GACAAGAGCC
481      ATGAAGATCC TCATGAAAGT TGGACAAGAT GCAAGTTCTG CTGGATCAGC CAGGAATCAC
541      GGTCCAACAA GACGTCCAGA GCTAGAAGCT GGTACAAATG GGAGAAGTTC AACAACAAGT
601      CCCTTTGTGA AGCCAAATCC AGGTTCTAGC ACCGATGGCA ACAGCGCGGG GCATTCCGGG
661      AACAATCTCC TGGGTTCCGA AGTGGCCTTA TTCGCAGGGA TCGCATCAGG ATGCATCATC
721      TTCATCGTCA TCATCATCAC TTTGGTGGTG CTGCTGCTCA AGTACCGCAG GAGACACCGC
781      AAACACTCTC CACAGCACAC GACCACGCTG TCTCTCAGCA CACTGGCCAC GCCCAAGCGA
841      GGTGGCAACA ACAATGGCTC GGAGCCCAGT GACGTTATCA TACCACTAAG GACTGCAGAC
901      AGCGTCTTCT GCCCGCACTA CGAGAAGGTC AGCGGGGACT ATGGGCACCC GGTGTACATC
961      GTGCAGGAGA TGCCCCCACA GAGTCCTGCC AACATTTACT ACAAGGTCTG A

 

Quality Control:cDNA sequencing results is available upon request.