EphrinB1 / EFNB1 Protein

Ephrin-B1 / EFNB1 / ELK-L Protein

Ephrin-B1 / EFNB1 / ELK-L Protein Product Information

• Synonym: EFNB1 , RP3-496G1.1 , CFND , CFNS, EFL3 , EPLG2 , Elk-L, LERK2, MGC8782
• Protein Construction: A DNA sequence encoding the human EFNB1 ( NP_004420.1 ) extracellular domain ( Met 1 - Gly 232 ) was was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus
• Source: Human
• Expression Host: Human Cells

Ephrin-B1 / EFNB1 / ELK-L Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA
  Immobilized mouse EphB3 at 2 μg/ml ( 100 μl/well ) can bind human EFNB1 Fc chimera with a linear ranger of 1.56 - 25 ng/ml
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 28
• Molecular Mass: The recombinant human EFNB1/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 458 amino acids and predicts a molecular mass of 51.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EFNB1/Fc monomer is approximately 62 kDa due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EFNB1 protein

Ephrin-B1 / EFNB1 / ELK-L Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Ephrin-B1 / EFNB1 / ELK-L Protein Description

The ephrins (Eph) and Eph receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. In molecular biology, ephrins and Eph receptors are components of cell signaling pathways involved in animal development, and implicated in some cancers.  Ephrin-B1, also known as LERK-2, ELK-L, EFL-3, Cek5-L, and STRA-1, is a member of the ephrin ligand family which binds members of the Eph receptor family. Ephrin-B1 has been shown to bind EphA3, EphB1, EphB2, EphB3, and EphB4. The extracellular domains of human and mouse ephrin-B1 share 94% amino acid identity.

References

1. Cerretti, DP. et al., 1995, Mol. Immunol. 32 (16): 1197-205.
2. Gale, NW. et al.,1996, Neuron.17 (1): 9-19.
3. Flanagan, J. et al., 1998, Annual Review of Neuroscience. 21: 309-45.
4. Holder, N. et al., 1999,  Development. 126 (10): 2033-44.
5. Wilkinson, DG. et al., 2001, Nat. Rev. Neurosci. 2 (3): 155-64. 
6. Murai, KK. et al., 2003, Journal of Cell Science. 116 (Pt 14): 2823-32.