Mouse EFNB1 Gene cDNA Clone / ORF Clone

EFNB1 cDNA Clone Datasheet

EFNB1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010110.4
• cDNA Size: 1038
• Gene Synonym: Epl2; EFL-3; Elk-L; Eplg2; Lerk2; Stra1; Cek5-L; LERK-2; Efnb1
• Source: Mouse

EFNB1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Ephrin-B1 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

EFNB1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EFNB1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EFNB1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCCGGC CTGGGCAGCG TTGGCTCAGC AAGTGGCTTG TGGCTATGGT CGTGCTGACG
61       CTGTGCCGGC TTGCCACGCC GTTGGCCAAG AACCTGGAGC CCGTGTCCTG GAGCTCTCTT
121      AACCCTAAGT TCCTAAGTGG GAAGGGCTTG GTGATCTACC CGAAGATTGG AGACAAGCTG
181      GACATCATCT GCCCCCGAGC AGAAGCAGGG CGGCCCTACG AGTACTACAA GCTGTACCTG
241      GTGCGGCCAG AGCAGGCGGC TGCTTGCAGC ACTGTGCTTG ATCCCAATGT ACTGGTCACT
301      TGCAACAAGC CACACCAGGA AATCCGCTTC ACCATCAAGT TCCAAGAGTT CAGCCCCAAC
361      TACATGGGCC TGGAATTCAA AAAGTACCAC GATTACTACA TTACATCAAC GTCCAATGGG
421      AGCTTGGAGG GACTGGAGAA CCGGGAGGGA GGTGTGTGTC GCACCCGCAC TATGAAGATC
481      GTTATGAAGG TTGGGCAAGA TCCAAATGCT GTGACACCCG AGCAGTTGAC TACCAGCCGG
541      CCAAGCAAGG AGTCAGACAA CACTGTCAAG ACAGCCACAC AGGCTCCTGG TCGGGGATCC
601      CAGGGTGACT CTGACGGCAA GCATGAGACT GTGAACCAGG AAGAGAAGAG TGGCCCAGGT
661      GCAGGTGGCG GTGGCAGCGG GGACTCTGAC AGCTTCTTCA ACTCCAAGGT AGCATTGTTC
721      GCAGCCGTCG GCGCCGGCTG TGTCATCTTC CTGCTCATCA TCATCTTCTT GACAGTCCTA
781      CTACTCAAGC TCCGCAAGCG CCATCGCAAG CATACACAGC AGCGGGCGGC TGCCCTCTCG
841      CTCAGTACCC TAGCCAGCCC CAAAGGGGGT AGTGGTACAG CGGGCACCGA GCCCAGCGAC
901      ATCATCATCC CCTTACGGAC TACAGAGAAC AACTACTGCC CCCACTATGA GAAGGTGAGT
961      GGGGACTACG GGCATCCTGT CTACATCGTC CAGGAGATGC CCCCTCAGAG CCCGGCGAAC
1021     ATCTACTACA AGGTTTGA

 

Quality Control:cDNA sequencing results is available upon request.