Mouse EFNA5 / Ephrin-A5 Protein (Fc Tag)

Mouse Ephrin-A5 / EFNA5 Protein

Ephrin-A5 / EFNA5 Protein Product Information

• Synonym: AL-1, AV158822, EFL-5, Ephrin-A5, Epl7, LERK-7, RAGS
• Protein Construction: A DNA sequence encoding the mouse EFNA5 isoform long ( O08543-1 ) without the pro peptide ( Met 1 - Asn 203 ) was as fused with the Fc region of human IgG1 at the C-terminus
• Source: Mouse
• Expression Host: Human Cells

Ephrin-A5 / EFNA5 Protein QC Testing

• Purity: > 85% as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA
  Immobilized Mouse EPHA4 at 2 μg/ml ( 100 μl/well ) can bind mouse EFNA5-Fc with a linear ranger of 1.28 - 32 ng/ml
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 21
• Molecular Mass: The recombinant mouse EFNA5 / Fc is a disulfide-linked homodimer. The reduced monomer consists of 424 amino acids and has a predicted molecular mass of 48.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEFNA5 / Fc monomer is approximately 52 kDa due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EFNA5 protein

Ephrin-A5 / EFNA5 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Ephrin-A5 / EFNA5 Protein Description

Mouse ephrin-A5, also known as AL-1, RAGS, or LERK-7 ( ligand for eph-related kinases, 7 ), is a member of the ephrin ligand family which binds the members of Eph receptor subfamily of tyrosine kinases. Based on their structure and sequence relationships, ephrins are divided into ephrin-A and ephrin-B class, and ephrin-A members are anchored to the membrane by GPI linkage. Ephrin-A5 is expressed with the highest levels in human adult brain, heart, spleen, and ovary and human fetal brain, lung, and kidney. This ligand has been reported to bind Eph A2, Eph A3, Eph A4, Eph A5, Eph A6, Eph A7, Eph A8, and Eph B2 receptors. The interactions between Eph receptors and their ephrin ligands have been implicated in mediating developmental events, particularly in the nervous system through regulating cell migration and axon pathfinding. It has been reported that Ephrin-A5 prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation.
The ephrins (EPH) and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases (RTKs), and have been involved in a variety of biological processes, especially in the nervous system and in erythropoiesis, such as axon guidance and topographic map formation, synaptic plasticity, angiogenesis, and meanwhile have possible contributions to tumor growth and metastasis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class which are anchored to the membrane by a GPI linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. 14 receptors and 8 ligands have been reported in mammals.

References

1. Stein, E. et al., 1999, J. Neurosci. 19: 8885-93.
2. Wilkinson, D.G. 2001, Nat. Rev. Neurosci. 2: 155-164.
3. Cheng, N. et al., 2002, Cytokine. Growth. Factor. Rev. 13: 75-85.
4. Himanen, J.P. et al., 2004, Nat. Neurosci. 7: 501-509.
5. Eberhart, J. et al., 2004, J. Neurosci. 24: 1070-1078.
6. Irie, F. et al., 2008, Proc. Nat. Acad. Sci. 105: 12307-12.