EphrinA5 / EFNA5 Antibody (Antigen Affinity Purified)

Ephrin-A5 / EFNA5 / EPLG7 Antibody ( Antigen Affinity Purified )

Ephrin-A5 / EFNA5 / EPLG7 Antibody Product Information

• Immunogen: Recombinant human EphrinA5 protein ( Catalog#10192-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human EphrinA5 ( rhEphrinA5 ; Catalog#10192-H08H ; NP_001953.1 ; Met 1- Asn 203 ). EphrinA5 specific IgG was purified by human EphrinA5 affinity chromatography.

Ephrin-A5 / EFNA5 / EPLG7 Antibody Usage Guide

• Specificity: Human EphrinA5 / EFNA5 / EPLG7
• Western blot: This antibody can be used at 0.1μg/mL - 0.2 μg/mL with the appropriate secondary reagents to detect human EphrinA5. Using a DAB detection system, the detection limit for EphrinA5 is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human EphrinA5. The detection limit for Human EphrinA5 is 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Ephrin-A5 / EFNA5 / EPLG7 Antibody Background

Ephrin-A5, also known as AL-1, RAGS, or LERK-7 (ligand for eph-related kinases, 7), is a member of the ephrin ligand family which binds the members of Eph receptor subfamily of tyrosine kinases. Based on their structure and sequence relationships, ephrins are divided into ephrin-A and ephrin-B class, and ephrin-A members are anchored to the membrane by GPI linkage. Ephrin-A5 is expressed with the highest levels in human adult brain, heart, spleen, and ovary and human fetal brain, lung, and kidney. This ligand has been reported to bind Eph A2, Eph A3, Eph A4, Eph A5, Eph A6, Eph A7, Eph A8, and Eph B2 receptors. The interactions between Eph receptors and their ephrin ligands have been implicated in mediating developmental events, particularly in the nervous system through regulating cell migration and axon pathfinding. It has been reported that Ephrin-A5 prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation.

References

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2. Kozlosky, CJ. et al., 1997, Cytokine. 9: 540-549.
3. Pasquale, EB. et al., 1997, Curr. Opin.Cell Biol. 9: 608-615.
4. Himanen, JP. et al., 2004, Nat. Neurosci. 7: 501-509.
5. Eberhart, J. et al., 2004, J. Neurosci. 24: 1070-1078.