Mouse EFNA3 / Ephrin-A3 Protein (His Tag)

Mouse EFNA3 / Ephrin-A3 Protein

EFNA3 / Ephrin-A3 Protein Product Information

• Synonym: AW494418, EFL-2, Ehk1-L, Epl3, LERK-3, MGC129300, MGC129301
• Protein Construction: A DNA sequence encoding the mouse EFNA3 ( NP_034238.1 ) without the pro peptide ( Met 1 - Ser 205 ) was expressed, with a polyhistidine tag at the C-terminus
• Source: Mouse
• Expression Host: Human Cells

EFNA3 / Ephrin-A3 Protein QC Testing

• Purity: > 92% as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA
  Immobilized mouse EphrinA3 at 1 μg/ml ( 100 μl/well ) can bind mouse EPHA6 with a linear ranger of 6.25 - 400 ng/ml
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 23
• Molecular Mass: The recombinant mouse EFNA3 consists of 194 amino acids and has a predicted molecular mass of 22.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEFNA3 is approximately 38 kDa due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EFNA3 protein

EFNA3 / Ephrin-A3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EFNA3 / Ephrin-A3 Protein Description

The ephrins (EPH) and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases (RTKs), and have been involved in a variety of biological processes, especially in the nervous system and in erythropoiesis, such as axon guidance and topographic map formation, synaptic plasticity, angiogenesis, and meanwhile have possible contributions to tumor growth and metastasis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class which are anchored to the membrane by a GPI linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. 14 receptors and 8 ligands have been reported in mammals.
Mouse ephrin-A3, also known as EPH-related receptor tyrosine kinase ligand 3, EFNA3 and LERK-3, is a cell membrane protein which belongs to the ephrin family. Ephrin-A3 / EFNA3 is expressed in brain, skeletal muscle, spleen, thymus, prostate, testis, ovary, small intestine, and peripheral blood leukocytes. EphrinA3 is capable of binding EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, and EphB1. The EphrinA3 ligand may interact with the EphA5 receptor to restrict entorhinal axon terminals in the outer molecular layer of the dentate gyrus. Furthermore, Research evidences indicated that ephrin-A3, but not other ligands, binds to heparan sulfate, and the presence of cell surface heparan sulfate is required for the full biological activity of ephrin-A3.

References

1. Zhou, R. Pharmacol. Ther. 77: 151-81.
2. Stein, E. et al., 1999, J. Neurosci. 19: 8885-93.
3. Cheng, N. et al., 2002, Cytokine. Growth. Factor. Rev. 13: 75-85.
4. Irie, F. et al., 2008, Proc. Nat. Acad. Sci. 105: 12307-12.