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Human EphrinA1 Gene cDNA Clone / ORF Clone

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HG10882-M
  YES          
Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_004428.2

cDNA Size:   618

Synonym:  B6 1; EFL1; ECKLG; EPLG1; LERK1; TNFAIP4

Source:  Human. cDNA Description:  ORF Clone of Homo sapiens ephrin-A1 DNA.

Vector:  Vector pMD18-T Simple

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for the point mutation 476A/T resulting in the amino acid 159Asp substitution by Val. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
  1. Remove the marked edge in the center of the spot .
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to a heating block at 95℃ for 15–30 min .
  4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:

1        ATGGAGTTCC TCTGGGCCCC TCTCTTGGGT CTGTGCTGCA GTCTGGCCGC TGCTGATCGC
61 CACACCGTCT TCTGGAACAG TTCAAATCCC AAGTTCCGGA ATGAGGACTA CACCATACAT
121 GTGCAGCTGA ATGACTACGT GGACATCATC TGTCCGCACT ATGAAGATCA CTCTGTGGCA
181 GACGCTGCCA TGGAGCAGTA CATACTGTAC CTGGTGGAGC ATGAGGAGTA CCAGCTGTGC
241 CAGCCCCAGT CCAAGGACCA AGTCCGCTGG CAGTGCAACC GGCCCAGTGC CAAGCATGGC
301 CCGGAGAAGC TGTCTGAGAA GTTCCAGCGC TTCACACCTT TCACCCTGGG CAAGGAGTTC
361 AAAGAAGGAC ACAGCTACTA CTACATCTCC AAACCCATCC ACCAGCATGA AGACCGCTGC
421 TTGAGGTTGA AGGTGACTGT CAGTGGCAAA ATCACTCACA GTCCTCAGGC CCATGTCAAT
481 CCACAGGAGA AGAGACTTGC AGCAGATGAC CCAGAGGTGC GGGTTCTACA TAGCATCGGT
541 CACAGTGCTG CCCCACGCCT CTTCCCACTT GCCTGGACTG TGCTGCTCCT TCCACTTCTG
601 CTGCTGCAAA CCCCGTGA

Quality Control:   cDNA sequencing results is available upon request.

 

Ephrin-A1/EFNA1 related areas, pathways, and other information