Ephrin-A1 Matched ELISA Antibody Pair Set,Human

Cat: SEK10882
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Ephrin-A1 Matched ELISA Antibody Pair Set,Human General Information
Product name
Ephrin-A1 Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human Ephrin-A1
Assay range
39.06-2500
Materials provided
1. Capture Antibody 0.5 mg/mL of mouse anti-EFNA1 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10882-MM01)
2. Detection Antibody 1 mg/mL rabbit anti-EFNA1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.2 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10882-R114)
3. Standard Each vial contains 140 ng of recombinant EFNA1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
Product overview
This Ephrin-A1 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human Ephrin-A1 . It contains Human Ephrin-A1 capture antibody, Human Ephrin-A1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Ephrin-A1 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Ephrin-A1 Matched ELISA Antibody Pair Set,Human Images
Human Ephrin-A1/EFNA1 ELISA Pair Set Standard Curve 13886
Ephrin-A1 Matched ELISA Antibody Pair Set,Human Alternative Names
B61 Matched ELISA Antibody Pair Set, Human;ECKLG Matched ELISA Antibody Pair Set, Human;EFL1 Matched ELISA Antibody Pair Set, Human;EFNA1 Matched ELISA Antibody Pair Set, Human;EPLG1 Matched ELISA Antibody Pair Set, Human;LERK-1 Matched ELISA Antibody Pair Set, Human;LERK1 Matched ELISA Antibody Pair Set, Human;TNFAIP4 Matched ELISA Antibody Pair Set, Human
Ephrin-A1 Background Information

EPH-related receptor tyrosine kinase ligand 1 (abbreviated as Ephrin-A1) also known as ligand of eph-related kinase 1 or EFNA1, is a member of the ephrin (EPH) family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A1/EFNA1 and its Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. Ephrin-A1 and one of its receptor EphA2 were expressed in xenograft endothelial cells and also tumor cells and play a role in human cancers, at least in part by influencing tumor neovascularization.

Full Name
ephrin-A1
References
  • Deroanne C, et al. (2003) EphrinA1 inactivates integrin-mediated vascular smooth muscle cell spreading via the Rac/PAK pathway. J Cell Sci. 116(7): 1367-76.
  • Ojima T, et al. (2006) EphrinA1 inhibits vascular endothelial growth factor-induced intracellular signaling and suppresses retinal neovascularization and blood-retinal barrier breakdown. Am J Pathol. 168(1): 331-9.
  • Wu D, et al. (2004) Prognostic value of EphA2 and EphrinA-1 in squamous cell cervical carcinoma. Gynecol Oncol. 94(2): 312-9.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    Ephrin-A1 ELISA Kit, Human Cell supernatant KIT10882
    info info
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