+86-400-890-9989
Product Catalog
> ELISA > ELISA Pair Set > Ephrin-A1 / EFNA1 ELISA Pair Set, Human ELISA Pair Set Ephrin-A1 / EFNA1 ELISA Pair Set, Human ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK10882 |
|
YES | 
|
    |
Human Ephrin-A1 / EFNA1 ELISA Pair Set
 |
Order or Inquire for Ephrin-A1 / EFNA1 ELISA Pair Set |
|
|
High affinity Human Ephrin-A1 / EFNA1 ELISA Pair Set |
|
|
Detection limit - 39 pg/ml |
|
|
Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
|
|
|
|
|
No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
|
Substrate solution was not added |
Add substrate solution and continue |
|
|
Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
|
|
Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
|
Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
|
|
Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
|
|
Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
|
|
Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
|
Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
|
|
High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
|
Increase cycles of washes and soaking time between washes |
||
|
TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
|
|
Materials were contaminated |
Use clean plates, tubes and pipettes tips |
|
|
Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
|
The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human Ephrin-A1 / EFNA1 ELISA Pair Set Related Products & Topics
Related Areas:
Proteins:
Antibodies:
Background
The ephrins (Eph) and Eph receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. In molecular biology , ephrins and Eph receptors are components of cell signaling pathways involved in animal development, and implicated in some cancers. Eph receptors are classified as receptor tyrosine kinases (RTKs), and form the largest sub-family of RTKs. Almost all Eph receptors are expressed during various well defined stages of development in assorted locations and concentrations. Ephrin-A1, also known as B61, LERK-1, and EFL-1, is a member of the ephrin ligand family which binds members of the Eph receptor family. Ephrin-A1 has been shown to bind EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1. The extracellular domains of human and mouse ephrin-A1 share 85% amino acid identity.
