EphrinA1 / EFNA1 Antibody (Mouse Monoclonal Antibody)

EphrinA1 / EFNA1 Antibody

EphrinA1 / EFNA1 Antibody Product Information

• Immunogen: Recombinant human EFNA1 protein ( Catalog#10882-H08H )
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 3F7H7F6
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human EphrinA1 ( rh EphrinA1 ; Catalog#10882-H08H ; NP_004419.2 ; Met 1 - Ser 182 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

EphrinA1 / EFNA1 Antibody Usage Guide

• Specificity: Human EphrinA1 / EFNA1
• No cross-reactivity in WB and ELISA with
  Human EphrinA5 / EFNA5
  Human EphrinA3 / EFNA3
  Human cell lysate (293 cell line)
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human EFNA1 in WB. Using a DAB detection system, the detection limit for human EFNA1 is approximately 4 ng/lane under non-reducing conditions 2 ng/lane and reducing conditions
• Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human EFNA1. The detection limit for human EFNA1 is 0.078 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

EphrinA1 / EFNA1 Antibody Background

The ephrins (Eph) and Eph receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. In molecular biology , ephrins and Eph receptors are components of cell signaling pathways involved in animal development, and implicated in some cancers. Eph receptors are classified as receptor tyrosine kinases (RTKs), and form the largest sub-family of RTKs. Almost all Eph receptors are expressed during various well defined stages of development in assorted locations and concentrations. Ephrin-A1, also known as B61, LERK-1, and EFL-1, is a member of the ephrin ligand family which binds members of the Eph receptor family. Ephrin-A1 has been shown to bind EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1. The extracellular domains of human and mouse ephrin-A1 share 85% amino acid identity. 

References

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7. Murai, KK. et al., 2003, Journal of Cell Science. 116 (Pt 14): 2823-32.