EphB6 / EPHB6 Protein (His Tag)

Ephrin Type-B Receptor 6 ( EphB6 / EPHB6 ) Protein

EphB6 / EPHB6 Protein Product Information

• Synonym: EphB6
• Protein Construction: The extracellular domain ( Met 1 - Ser 579 ) of human EphB6 ( NP_004436.1 ) precursor was expressed, fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

EphB6 / EPHB6 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized recombinant human EphB6 at 10 μg/ml (100 μl/well) can bind human EphrinB1 with a linear range of 32-800 ng/ml.
  3. Immobilized recombinant human EphB6 at 10 μg/ml (100 μl/well) can bind human EphrinB2 with a linear range of 1.28-32 ng/ml.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 17
• Molecular Mass: The secreted recombinant human EphB6 consists of 574 amino acids and has a calculated molecular mass of 61.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEphB6 is approximately 60-70 kDa due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EphB6 protein

EphB6 / EPHB6 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EphB6 / EPHB6 Protein Description

Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family, and EphB6 binding to ephrin-B ligands is a unique member in that its kinase domain contains several alterations in conserved amino acids and is catalytically inactive. It is indicated that activated by an Src family kinase, EphB6 can act as both positive and negative regulators in the process of cell adhesion and migration,The large family of Ephrin receptors and their ligands , the ephrins , mediate numerous developmental processes, particularly the establishment of regional pattern in the nervous system. Furthermore, the Eph receptors have been suggested to play important roles in tumor formation, prognosis, and possible gene-therapeutic approaches, and the reduction of EphB6 gene expression is associated with the progression of metastatic diseases.

References

1. Flanagan, J.G. et al., 1998, Annu. Rev. Neurosci. 21: 309-45.
2. Matsuoka, H. et al., 2005, J. Biol. Chem. 280: 29355-63.
3. Holder, N. et al., 1999, Development. 126: 2033-44.
4. Cheng, N. et al., 2002, Cytokine. Growth. Factor. Rev. 13: 75-85.
5. Hafner,C. et al., 2003, Int. J.Oncol. 23: 1553-9.
6. Fox, B.P. et al., 2006, Biochem.Biophys.Res.Commun.340: 268-76.