EphB6 / EPHB6 Protein

EPH receptor B6 Protein Datasheet

EphB6 Protein Product Information

• EphB6 Synonym: EphB6
• Protein Construction: The mature form of human EphB6 (NP_004436.1) extracellular domain (Met 1- Ser 579) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.
• Source: Human
• Expression Host: Human Cells

EphB6 Protein QC Testing

• Purity: > 95%, as determined by SDS-PAGE and SEC-HPLC Analysis
• Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 17
• Molecular Mass: The recombinant mature human EphB6 consists of 569 amino acids and has a calculated molecular mass of 60.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEphB6 is approximately 72kDa due to glycosylation.
• Formulation: Lyophilized from sterile 100mM NaCl, 50mM Tris, pH7.5
• SDS-PAGE:

EphB6 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage and usage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EphB6 Protein Description

Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family, and EphB6 binding to ephrin-B ligands is a unique member in that its kinase domain contains several alterations in conserved amino acids and is catalytically inactive. It is indicated that activated by an Src family kinase, EphB6 can act as both positive and negative regulators in the process of cell adhesion and migration,The large family of Ephrin receptors and their ligands , the ephrins , mediate numerous developmental processes, particularly the establishment of regional pattern in the nervous system. Furthermore, the Eph receptors have been suggested to play important roles in tumor formation, prognosis, and possible gene-therapeutic approaches, and the reduction of EphB6 gene expression is associated with the progression of metastatic diseases.

References

1. Flanagan, J.G. et al., 1998, Annu. Rev. Neurosci. 21: 309-345.
2. Matsuoka, H. et al., 2005, J. Biol. Chem. 280: 29355-29363.
3. Holder, N. et al., 1999, Development. 126: 2033-2044.
4. Cheng, N. et al., 2002, Cytokine. Growth. Factor. Rev. 13: 75-85.
5. Hafner,C. et al., 2003, Int. J.Oncol. 23: 1553-1559.
6. Fox, B.P. et al., 2006, Biochem.Biophys.Res.Commun.340: 268-276.