Cynomolgus EphB6 / EPHB6 Protein

Eph receptor B6 Protein Datasheet

EphB6 / EPHB6 Protein Price Inquiry ( Available Sizes )

• ≥1mg Bulk:  Inquiring Price

EphB6 / EPHB6 Protein Product Information

• Synonym: EPHB6
• Protein Construction: A DNA sequence encoding the cynomolgus EPHB6 (Met1-Ser591) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Cynomolgus
• Expression Host: Human Cells

EphB6 / EPHB6 Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 32
• Molecular Mass: The recombinant cynomolgus EPHB6 is a disulfide-linked homodimer. The reduced monomer comprises 801 amino acids and has a calculated molecular mass of 86.9 KDa.The apparent molecular mass of the protein is approximately 97 KDa respectively in SDS-PAGE.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EphB6 / EPHB6 protein

EphB6 / EPHB6 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EphB6 / EPHB6 Protein Description

Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family, and EphB6 binding to ephrin-B ligands is a unique member in that its kinase domain contains several alterations in conserved amino acids and is catalytically inactive. It is indicated that activated by an Src family kinase, EphB6 can act as both positive and negative regulators in the process of cell adhesion and migration,The large family of Ephrin receptors and their ligands , the ephrins , mediate numerous developmental processes, particularly the establishment of regional pattern in the nervous system. Furthermore, the Eph receptors have been suggested to play important roles in tumor formation, prognosis, and possible gene-therapeutic approaches, and the reduction of EphB6 gene expression is associated with the progression of metastatic diseases.

References

1. Flanagan JG. et al., 1998, Annu Rev Neurosci. 21: 309-45.
2. Matsuoka H. et al., 2005, J Biol Chem. 280: 29355-63.
3. Holder N. et al., 1999, Development. 126: 2033-44.
4. Cheng N. et al., 2002, Cytokine Growth Factor Rev. 13: 75-85.
5. Hafner C. et al., 2003, Int J Oncol. 23: 1553-9.
6. Fox BP. et al., 2006, Biochem Biophys Res Commun.340: 268-76.