Human EphB4 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10235-M	Cloning vector ($95) pCMV / hygro ($345) pCMV / hygro - HA ($345) pCMV / hygro - His ($345) pCMV / hygro - Myc ($345) pCMV / hygro - FLAG ($345)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

EphB4 cDNA Clone Datasheet

EphB4 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_004444.4
cDNA Size:	2964
Gene Synonym:	HTK, MYK1, TYRO11
Source:	Human

EphB4 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens EPH receptor B4 DNA.
Vector:	Cloning Vector: pMD18-T Simple Vector. Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 1314 T/C，1752 A/G，1890 C/T, 2934 T/C not causing the amino acid variation.

EphB4 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EphB4 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EphB4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGCTCC GGGTGCTGCT CTGCTGGGCT TCGTTGGCCG CAGCTTTGGA AGAGACCCTG
61       CTGAACACAA AATTGGAAAC TGCTGATCTG AAGTGGGTGA CATTCCCTCA GGTGGACGGG
121      CAGTGGGAGG AACTGAGCGG CCTGGATGAG GAACAGCACA GCGTGCGCAC CTACGAAGTG
181      TGTGACGTGC AGCGTGCCCC GGGCCAGGCC CACTGGCTTC GCACAGGTTG GGTCCCACGG
241      CGGGGCGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTCGAGTG CCTGTCCCTG
301      CCTCGGGCTG GGCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTATGA GAGCGATGCG
361      GACACGGCCA CGGCCCTCAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG
421      GTGGCCGCGG AGCATCTCAC CCGGAAGCGC CCTGGGGCCG AGGCCACCGG GAAGGTGAAT
481      GTCAAGACGC TGCGTCTGGG ACCGCTCAGC AAGGCTGGCT TCTACCTGGC CTTCCAGGAC
541      CAGGGTGCCT GCATGGCCCT GCTATCCCTG CACCTCTTCT ACAAAAAGTG CGCCCAGCTG
601      ACTGTGAACC TGACTCGATT CCCGGAGACT GTGCCTCGGG AGCTGGTTGT GCCCGTGGCC
661      GGTAGCTGCG TGGTGGATGC CGTCCCCGCC CCTGGCCCCA GCCCCAGCCT CTACTGCCGT
721      GAGGATGGCC AGTGGGCCGA ACAGCCGGTC ACGGGCTGCA GCTGTGCTCC GGGGTTCGAG
781      GCAGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCCCAGG GCACCTTCAA GCCCCTGTCA
841      GGAGAAGGGT CCTGCCAGCC ATGCCCAGCC AATAGCCACT CTAACACCAT TGGATCAGCC
901      GTCTGCCAGT GCCGCGTCGG GTACTTCCGG GCACGCACAG ACCCCCGGGG TGCACCCTGC
961      ACCACCCCTC CTTCGGCTCC GCGGAGCGTG GTTTCCCGCC TGAACGGCTC CTCCCTGCAC
1021     CTGGAATGGA GTGCCCCCCT GGAGTCTGGT GGCCGAGAGG ACCTCACCTA CGCCCTCCGC
1081     TGCCGGGAGT GCCGACCCGG AGGCTCCTGT GCGCCCTGCG GGGGAGACCT GACTTTTGAC
1141     CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGTGGTTC GAGGGCTACG TCCTGACTTC
1201     ACCTATACCT TTGAGGTCAC TGCATTGAAC GGGGTATCCT CCTTAGCCAC GGGGCCCGTC
1261     CCATTTGAGC CTGTCAATGT CACCACTGAC CGAGAGGTAC CTCCTGCAGT GTCCGACATC
1321     CGGGTGACGC GGTCCTCACC CAGCAGCTTG AGCCTGGCCT GGGCTGTTCC CCGGGCACCC
1381     AGTGGGGCTG TGCTGGACTA CGAGGTCAAA TACCATGAGA AGGGCGCCGA GGGTCCCAGC
1441     AGCGTGCGGT TCCTGAAGAC GTCAGAAAAC CGGGCAGAGC TGCGGGGGCT GAAGCGGGGA
1501     GCCAGCTACC TGGTGCAGGT ACGGGCGCGC TCTGAGGCCG GCTACGGGCC CTTCGGCCAG
1561     GAACATCACA GCCAGACCCA ACTGGATGAG AGCGAGGGCT GGCGGGAGCA GCTGGCCCTG
1621     ATTGCGGGCA CGGCAGTCGT GGGTGTGGTC CTGGTCCTGG TGGTCATTGT GGTCGCAGTT
1681     CTCTGCCTCA GGAAGCAGAG CAATGGGAGA GAAGCAGAAT ATTCGGACAA ACACGGACAG
1741     TATCTCATCG GGCATGGTAC TAAGGTCTAC ATCGACCCCT TCACTTATGA AGACCCTAAT
1801     GAGGCTGTGA GGGAATTTGC AAAAGAGATC GATGTCTCCT ACGTCAAGAT TGAAGAGGTG
1861     ATTGGTGCAG GTGAGTTTGG CGAGGTGTGT CGGGGGCGGC TCAAGGCCCC AGGGAAGAAG
1921     GAGAGCTGTG TGGCAATCAA GACCCTGAAG GGTGGCTACA CGGAGCGGCA GCGGCGTGAG
1981     TTTCTGAGCG AGGCCTCCAT CATGGGCCAG TTCGAGCACC CCAATATCAT CCGCCTGGAG
2041     GGCGTGGTCA CCAACAGCAT GCCCGTCATG ATTCTCACAG AGTTCATGGA GAACGGCGCC
2101     CTGGACTCCT TCCTGCGGCT AAACGACGGA CAGTTCACAG TCATCCAGCT CGTGGGCATG
2161     CTGCGGGGCA TCGCCTCGGG CATGCGGTAC CTTGCCGAGA TGAGCTACGT CCACCGAGAC
2221     CTGGCTGCTC GCAACATCCT AGTCAACAGC AACCTCGTCT GCAAAGTGTC TGACTTTGGC
2281     CTTTCCCGAT TCCTGGAGGA GAACTCTTCC GATCCCACCT ACACGAGCTC CCTGGGAGGA
2341     AAGATTCCCA TCCGATGGAC TGCCCCGGAG GCCATTGCCT TCCGGAAGTT CACTTCCGCC
2401     AGTGATGCCT GGAGTTACGG GATTGTGATG TGGGAGGTGA TGTCATTTGG GGAGAGGCCG
2461     TACTGGGACA TGAGCAATCA GGACGTGATC AATGCCATTG AACAGGACTA CCGGCTGCCC
2521     CCGCCCCCAG ACTGTCCCAC CTCCCTCCAC CAGCTCATGC TGGACTGTTG GCAGAAAGAC
2581     CGGAATGCCC GGCCCCGCTT CCCCCAGGTG GTCAGCGCCC TGGACAAGAT GATCCGGAAC
2641     CCCGCCAGCC TCAAAATCGT GGCCCGGGAG AATGGCGGGG CCTCACACCC TCTCCTGGAC
2701     CAGCGGCAGC CTCACTACTC AGCTTTTGGC TCTGTGGGCG AGTGGCTTCG GGCCATCAAA
2761     ATGGGAAGAT ACGAAGAAAG TTTCGCAGCC GCTGGCTTTG GCTCCTTCGA GCTGGTCAGC
2821     CAGATCTCTG CTGAGGACCT GCTCCGAATC GGAGTCACTC TGGCGGGACA CCAGAAGAAA
2881     ATCTTGGCCA GTGTCCAGCA CATGAAGTCC CAGGCCAAGC CGGGAACCCC GGGCGGGACA
2941     GGAGGACCGG CCCCGCAGTA CTGA

Quality Control:cDNA sequencing results is available upon request.