Mouse EPHB4 Gene cDNA Clone / ORF Clone

EPHB4 cDNA Clone Datasheet

EPHB4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010144.6
• cDNA Size: 2964
• Gene Synonym: Htk; MDK2; Myk1; Tyro11; AI042935; Ephb4
• Source: Mouse

EPHB4 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Eph receptor B4 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for four point mutations: 861 A/G, 1077 A/T, 2565T/C and 2718 T/C not causing the amino acid variation.

EPHB4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EPHB4 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EPHB4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGCTCC GAGCGCTGCT GTGCTGGGCT TCCCTCGCCA CTGCTTTAGA AGAGACCCTG
61       TTGAACACAA AACTGGAAAC GGCGGATCTG AAATGGGTGA CTTACCCTCA GGCAGAGGGC
121      CAGTGGGAGG AGCTAAGCGG CCTGGATGAG GAACAGCACA GCGTCCGCAC CTATGAGGTG
181      TGCGACATGA AGCGTCCAGG GGGCCAGGCT CACTGGCTGC GCACTGGCTG GGTCCCAAGG
241      CGAGGTGCTG TCCACGTGTA TGCCACGATA CGCTTCACCA TGATGGAATG CCTGTCCCTG
301      CCGAGGGCCA GTCGCTCCTG CAAGGAGACA TTCACTGTCT TCTATTACGA GAGCGAAGCT
361      GATACGGCCA CGGCCCATAC GCCCGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACA
421      GTGGCCGCAG AACATCTGAC TCGGAAGCGC CCTGGAGCTG AAGCCACAGG GAAAGTTAAT
481      ATCAAGACGC TGCGCCTGGG TCCTCTCAGC AAAGCTGGCT TCTACCTGGC TTTCCAGGAC
541      CAAGGAGCCT GCATGGCTCT GCTCTCCCTG CATCTCTTTT ACAAGAAGTG CTCCTGGCTG
601      ATCACGAACT TGACCTACTT CCCCGAGACG GTGCCTCGGG AGCTCGTGGT GCCGGTGGCA
661      GGTAGCTGCG TGGCCAACGC GGTCCCTACC GCCAACCCCA GCCCCAGCCT CTACTGCCGG
721      GAAGATGGTC AATGGGCTGA GCAGCAGGTC ACGGGCTGCA GCTGCGCGCC AGGGTACGAG
781      GCTGCGGAAA GCAACAAAGT ATGCAGAGCC TGTGGCCAGG GAACCTTCAA GCCCCAAATA
841      GGAGACGAGT CCTGCCTGCC GTGCCCAGCC AACAGCCACT CGAATAACAT TGGGTCTCCT
901      GTCTGCCTGT GTCGAATTGG GTATTACCGG GCCCGCTCAG ACCCCCGGAG TTCACCTTGC
961      ACTACCCCAC CCTCTGCTCC AAGAAGCGTG GTTCACCATT TGAATGGTTC CACCCTGCGC
1021     CTGGAATGGA GTGCTCCCCT TGAGTCCGGA GGCCGAGAGG ACCTCACTTA TGCTGTTCGC
1081     TGCCGAGAGT GCCGTCCTGG GGGTTCCTGC TTGCCCTGTG GGGGCGACAT GACCTTCGAC
1141     CCCGGTCCTC GAGACCTGGT TGAGCCCTGG GTGGCAATCC GAGGGCTGCG TCCTGATGTC
1201     ACCTATACCT TTGAGGTTGC TGCTTTGAAT GGTGTGTCTA CCTTAGCCAC TGGACCACCT
1261     CCTTTTGAGC CTGTCAATGT CACCACTGAC CGTGAGGTGC CTCCTGCAGT GTCTGACATC
1321     CGAGTGACTC GGTCGTCACC CAGCAGCTTG ATCCTGTCAT GGGCTATCCC CAGAGCACCC
1381     AGTGGGGCCG TGCTGGACTA CGAGGTCAAG TATCATGAGA AGGGCGCAGA GGGCCCCAGC
1441     AGTGTTCGTT TCCTGAAGAC ATCAGAAAAC CGAGCTGAGC TCCGGGGGCT GAAGCGGGGA
1501     GCCAGCTATC TGGTCCAGGT ACGCGCACGG TCCGAGGCTG GCTACGGTCC CTTCGGCCAG
1561     GAGCATCACA GTCAGACTCA ACTGGATGAG AGCGAGAGCT GGCGGGAGCA GCTGGCCCTG
1621     ATTGCAGGCA CTGCGGTTGT GGGTGTGGTC CTGGTCCTGG TGGTCGTCAT CATTGCAGTT
1681     CTCTGCCTCA GGAAGCAGAG CAATGGGAGG GAAGTTGAGT ACTCGGATAA GCATGGGCAG
1741     TATCTCATCG GGCACGGTAC CAAGGTCTAC ATTGATCCTT TTACTTACGA AGACCCTAAT
1801     GAGGCAGTGA GGGAATTTGC CAAAGAGATC GATGTCTCCT ATGTCAAGAT TGAAGAGGTA
1861     ATTGGTGCAG GTGAGTTCGG CGAGGTGTGC CGGGGTCGGC TGAAGGCACC AGGGAAAAAG
1921     GAGAGCTGTG TGGCCATCAA GACTCTGAAG GGTGGCTACA CCGAGCGCCA GAGGCGTGAG
1981     TTCCTGAGCG AGGCCTCCAT CATGGGCCAG TTCGAGCATC CCAACATCAT CCGCCTCGAG
2041     GGCGTGGTCA CCAACAGTGT GCCGGTTATG ATCCTCACGG AATTCATGGA GAACGGAGCC
2101     CTGGACTCCT TCCTGCGGCT GAACGACGGG CAGTTCACAG TCATCCAGCT GGTGGGCATG
2161     CTGAGGGGCA TCGCCTCGGG CATGCGGTAC CTGGCTGAAA TGAGCTATGT CCACCGAGAC
2221     CTGGCTGCTC GGAACATCTT GGTCAACAGT AACCTGGTCT GCAAGGTGTC CGACTTTGGC
2281     CTCTCCAGAT TCTTGGAGGA GAACTCCTCT GATCCCACCT ACACAAGTTC CCTGGGAGGC
2341     AAGATTCCCA TCCGATGGAC CGCCCCTGAA GCCATTGCCT TCAGGAAGTT CACCTCTGCC
2401     AGTGATGCCT GGAGCTATGG GATCGTCATG TGGGAGGTCA TGTCTTTTGG GGAACGGCCA
2461     TACTGGGACA TGAGCAACCA GGATGTGATC AATGCCATTG AACAGGACTA CCGGCTGCCT
2521     CCTCCTCCAG ACTGCCCCAC CTCCCTCCAC CAGCTCATGC TGGACTGTTG GCAGAAGGAC
2581     CGGAATGCCC GGCCCCGCTT TCCCCAGGTG GTCAGCGCTC TGGACAAGAT GATCCGGAAT
2641     CCCGCTAGCC TCAAAATCGT GGCCAGGGAG AATGGCGGGG CCTCACATCC ACTCTTGGAC
2701     CAACGGCAGC CTCACTACTC TGCTTTCGGT TCTGTGGGCG AGTGGCTTCG AGCCATCAAG
2761     ATGGGAAGAT ACGAGGAAAG TTTTGCAGCG GCTGGATTCG GCTCCTTTGA GGTGGTCAGT
2821     CAGATCTCTG CCGAGGACCT TCTCCGAATT GGAGTCACTC TGGCAGGACA CCAGAAGAAA
2881     ATCTTGGCCA GTGTGCAGCA TATGAAGTCC CAAGCTAAGC CAGGAGCCCC TGGTGGGACA
2941     GGGGGACCAG CCCAGCAGTT CTGA

 

Quality Control:cDNA sequencing results is available upon request.