After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

EphB4 Antibody (FITC), Mouse MAb

DatasheetReviewsRelated ProductsProtocols
Human EphB4 Antibody Product Information
Immunogen:Recombinant Human EphB4 protein (Catalog#10235-H08H)
Clone ID:04
Ig Type:Mouse IgG1
Concentration:10 μl/Test, 0.1 mg/ml
Formulation:Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EphB4 (rhEphB4; Catalog#10235-H08H; NP_004435.3; Met 1-Ala 539) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Human EphB4 Antibody Usage Guide
Specificity:Human EphB4No cross-reactivity in WB and ELISA with
Human cell lysate (293 cell line)
Application:ICC/IF, FCM, IF

ICC/IF: 5-20 μg/mL

Storage:This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Human EphB4 Antibody IF Application Image
[Click to enlarge image]
Immunofluorescence staining of Human EphB4 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human EphB4 monoclonal antibody (FITC-conjugated, 10 µg/ml) at 37℃ 1 hour. Positive staining was localized to plasma membrane.
EphB4 Antibody (FITC), Mouse MAb, Immunofluorescence
Human EphB4 Antibody FC Application Image
[Click to enlarge image]
Profile of anti-EphB4 reactivity on MCF-7 cells analyzed by flow cytometry.
EphB4 Antibody (FITC), Mouse MAb, Flow cytometric
Other EphB4 Antibody Products
EphB4/Eph Receptor B4 Background

Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.

Human EphB4/Eph Receptor B4 References
  • Davalos V, et al. (2006) EPHB4 and survival of colorectal cancer patients. Cancer Res. 66(18): 8943-8.
  • Zhao C, et al. (2006) Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis. Cell Metab. 4(2): 111-21.
  • Kertesz N, et al. (2006) The soluble extracellular domain of EphB4 (sEphB4) antagonizes EphB4-EphrinB2 interaction, modulates angiogenesis, and inhibits tumor growth. Blood. 107(6): 2330-8.
  • Noren NK, et al. (2007) Paradoxes of the EphB4 receptor in cancer. Cancer Res. 67(9): 3994-7.
  • Taylor AC, et al. (2007) EphB4 expression along adult rat microvascular networks: EphB4 is more than a venous specific marker. Microcirculation. 14(3): 253-67.
  • Product nameProduct name
    Size / Price
    Catalog: 10235-MM04-F-50
    List Price:   (Save )
    Price:      [How to order]
    AvailabilityIn Stock
     Shipping instructions
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.