Anti-EphB4 Antibody

Cat: 10235-MM04
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All EphB4 Reagents
Anti-EphB4 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-EphB4 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human EphB4
Recombinant Human EphB4 protein (Catalog#10235-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EphB4 / HTK extracellular domain (rhEphB4; Catalog#10235-H08H; NP_004435.3; Met 1-Ala 539). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #04
Protein A
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-EphB4 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ICC-IF 1:20-1:100  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-EphB4 Antibody (Mouse Monoclonal antibody) Images
Human EphB4/Eph Receptor B4 Flow Cytometry (FC) 5182
Flow cytometric analysis of human EphB4 expression on MCF-7 cells. Cells were stained with purified anti-Human EphB4, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Human EphB4/Eph Receptor B4 Immunofluorescence(IF) 14804
Immunofluorescence staining of EPhB4 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human EPhB4 monoclonal antibody (1:30) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue).
Anti-EphB4 Antibody Alternative Names
Anti-HTK Antibody;Anti-MYK1 Antibody;Anti-TYRO11 Antibody
EphB4 Background Information

Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.

Full Name
EPH receptor B4
  • Davalos V, et al. (2006) EPHB4 and survival of colorectal cancer patients. Cancer Res. 66(18): 8943-8.
  • Zhao C, et al. (2006) Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis. Cell Metab. 4(2): 111-21.
  • Kertesz N, et al. (2006) The soluble extracellular domain of EphB4 (sEphB4) antagonizes EphB4-EphrinB2 interaction, modulates angiogenesis, and inhibits tumor growth. Blood. 107(6): 2330-8.
  • Noren NK, et al. (2007) Paradoxes of the EphB4 receptor in cancer. Cancer Res. 67(9): 3994-7.
  • Taylor AC, et al. (2007) EphB4 expression along adult rat microvascular networks: EphB4 is more than a venous specific marker. Microcirculation. 14(3): 253-67.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-EphB4 Antibody Rabbit Polyclonal ELISA,IHC-P 50582-RP02
    Anti-EphB4 Antibody Rabbit Monoclonal ELISA 50582-R039
    Anti-EphB4 Antibody Rabbit Polyclonal IHC-P 106504-T08
    Anti-EphB4 Antibody Rabbit Polyclonal ELISA 50582-RP01
    Anti-EphB4 Antibody Rabbit Monoclonal ELISA 10235-R047
    Anti-EphB4 Antibody Mouse Monoclonal ELISA 10235-MM12
    Anti-EphB4 Antibody Rabbit Polyclonal ELISA 10235-T16
    Anti-EphB4 Antibody Rabbit Polyclonal ELISA 10235-RP05
    Anti-EphB4 Antibody Rabbit Monoclonal ELISA 10235-RM02
    Anti-EphB4 Antibody (PE) Mouse Monoclonal FCM,ICC/IF,IF 10235-MM04-P
    Anti-EphB4 Antibody Rabbit Monoclonal ELISA,FCM 10235-R001
    Anti-EphB4 Antibody (APC) Mouse Monoclonal FCM 10235-MM04-A
    Anti-EphB4 Antibody (APC) Rabbit Monoclonal FCM 10235-R001-A
    Anti-EphB4 Antibody (FITC) Mouse Monoclonal FCM,ICC/IF,IF 10235-MM04-F
    Anti-EphB4 Antibody Rabbit Monoclonal ELISA 10235-R201
    Anti-EphB4 Antibody (PE) Rabbit Monoclonal FCM 10235-R001-P
    Anti-EphB4 Antibody Mouse Monoclonal ELISA 10235-MM05
    Anti-EphB4 Antibody (FITC) Rabbit Monoclonal FCM 10235-R001-F
    Anti-EphB4 Antibody Rabbit Polyclonal ELISA 10235-RP01
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