All EphB3 reagents are produced in house and quality controlled, including 8 EphB3 Antibody, 26 EphB3 Gene, 1 EphB3 IPKit, 3 EphB3 Lysate, 3 EphB3 Protein, 2 EphB3 qPCR. All EphB3 reagents are ready to use.
Recombinant EphB3 proteins are expressed by Baculovirus-Insect Cells, HEK293 Cells with fusion tags as N-GST & His, C-His.
EphB3antibodies are validated with different applications, which are ELISA, FCM, WB, IP, IHC-P.
EphB3cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each EphB3 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Expression host: Baculovirus-Insect Cells
Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.