EphB3 / HEK2 Antibody

EphB3 / HEK2 Antibody Datasheet

EphB3 / HEK2 Antibody Product Information

• Immunogen: Recombinant Mouse EphB3 / HEK2 protein (Catalog#50581-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 115
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse EphB3 / HEK2 (rM EphB3 / HEK2; Catalog#50581-M08H; NP_034273.1; Met 1-Thr 537).

EphB3 / HEK2 Antibody Usage Guide

• Specificity: Mouse EphB3 / HEK2
• No cross-reactivity in ELISA with

  Human LRPAP1

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse EphB3. The detection limit for Mouse EphB3 is approximately 0. 15625 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

EphB3 / HEK2 Antibody Background

Ephrin type-B receptor 3, EPH-like kinase 2, Tyrosine-protein kinase TYRO6, EPHB3, ETK2, HEK2 and TYRO6, is a single-pass type I membrane protein which belongs to theprotein kinase superfamily, tyr protein kinase family, ephrin receptor subfamily. EPHB3 contains twofibronectin type-III domains, oneprotein kinase domain and oneSAM (sterile alpha motif) domain. EphB3 is initially expressed in the sclerotome, but later is expressed predominantly in the dermatome. It is prominent expression is also detected in the developing heart, liver, posterior ventral limb bud mesenchyme, pharyngeal arches, and head mesenchyme. The EphB3 receptors are expressed during embryonic development in multiple regions of the central nervous system. The spatial profile of EphB3 receptors is co-localized to regions of the brain that had a high level of EphB3 receptor binding ligands. Its expression pattern suggests that EphB3 may play a role in the maintenance of mature neuronal connections or re-arrangement of synaptic connections during late stages of development. EphB3-ephrin-B interaction promotes MET by re-establishing epithelial cell-cell junctions and such an MET-promoting effect contributes to EphB3-mediated tumor suppression.

References

1. Baker,R.K. et al., 2001,Mech Dev. 104 (1-2): 129-32.
2. Willson,C.A. et al., 2003, Cell Transplant. 12 (3): 279-90.
3. Willson,C.A. et al., 2006, J Mol Histol. 37 (8-9): 369-80.
4. Risley,M. et al., 2009, Mech Dev. 126 (3-4): 230-9.
5. Chiu,S.T. et al., 2009, Carcinogenesis. 30 (9): 1475-86.