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Human EphB2 Gene cDNA Clone / ORF Clone
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10762-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_004442.6
cDNA Size: 2964
Synonym: DRT; ERK; CAPB; Hek5; PCBC; EPHT3; Tyro5; MGC87492; EPHB2
Source: Human.
cDNA Description: ORF Clone of Homo sapiens EPH receptor B2 DNA.
Vector: Vector pMD18-T Simple
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1377 G/A not causing the amino acid variation.
5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95℃ for 15-30 min.
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:
1 ATGGCTCTGC GGAGGCTGGG GGCCGCGCTG CTGCTGCTGC CGCTGCTCGC CGCCGTGGAA 61 GAAACGCTAA TGGACTCCAC TACAGCGACT GCTGAGCTGG GCTGGATGGT GCATCCTCCA 121 TCAGGGTGGG AAGAGGTGAG TGGCTACGAT GAGAACATGA ACACGATCCG CACGTACCAG 181 GTGTGCAACG TGTTTGAGTC AAGCCAGAAC AACTGGCTAC GGACCAAGTT TATCCGGCGC 241 CGTGGCGCCC ACCGCATCCA CGTGGAGATG AAGTTTTCGG TGCGTGACTG CAGCAGCATC 301 CCCAGCGTGC CTGGCTCCTG CAAGGAGACC TTCAACCTCT ATTACTATGA GGCTGACTTT 361 GACTCGGCCA CCAAGACCTT CCCCAACTGG ATGGAGAATC CATGGGTGAA GGTGGATACC 421 ATTGCAGCCG ACGAGAGCTT CTCCCAGGTG GACCTGGGTG GCCGCGTCAT GAAAATCAAC 481 ACCGAGGTGC GGAGCTTCGG ACCTGTGTCC CGCAGCGGCT TCTACCTGGC CTTCCAGGAC 541 TATGGCGGCT GCATGTCCCT CATCGCCGTG CGTGTCTTCT ACCGCAAGTG CCCCCGCATC 601 ATCCAGAATG GCGCCATCTT CCAGGAAACC CTGTCGGGGG CTGAGAGCAC ATCGCTGGTG 661 GCTGCCCGGG GCAGCTGCAT CGCCAATGCG GAAGAGGTGG ATGTACCCAT CAAGCTCTAC 721 TGTAACGGGG ACGGCGAGTG GCTGGTGCCC ATCGGGCGCT GCATGTGCAA AGCAGGCTTC 781 GAGGCCGTTG AGAATGGCAC CGTCTGCCGA GGTTGTCCAT CTGGGACTTT CAAGGCCAAC 841 CAAGGGGATG AGGCCTGTAC CCACTGTCCC ATCAACAGCC GGACCACTTC TGAAGGGGCC 901 ACCAACTGTG TCTGCCGCAA TGGCTACTAC AGAGCAGACC TGGACCCCCT GGACATGCCC 961 TGCACAACCA TCCCCTCCGC GCCCCAGGCT GTGATTTCCA GTGTCAATGA GACCTCCCTC 1021 ATGCTGGAGT GGACCCCTCC CCGCGACTCC GGAGGCCGAG AGGACCTCGT CTACAACATC 1081 ATCTGCAAGA GCTGTGGCTC GGGCCGGGGT GCCTGCACCC GCTGCGGGGA CAATGTACAG 1141 TACGCACCAC GCCAGCTAGG CCTGACCGAG CCACGCATTT ACATCAGTGA CCTGCTGGCC 1201 CACACCCAGT ACACCTTCGA GATCCAGGCT GTGAACGGCG TTACTGACCA GAGCCCCTTC 1261 TCGCCTCAGT TCGCCTCTGT GAACATCACC ACCAACCAGG CAGCTCCATC GGCAGTGTCC 1321 ATCATGCATC AGGTGAGCCG CACCGTGGAC AGCATTACCC TGTCGTGGTC CCAGCCAGAC 1381 CAGCCCAATG GCGTGATCCT GGACTATGAG CTGCAGTACT ATGAGAAGGA GCTCAGTGAG 1441 TACAACGCCA CAGCCATAAA AAGCCCCACC AACACGGTCA CCGTGCAGGG CCTCAAAGCC 1501 GGCGCCATCT ATGTCTTCCA GGTGCGGGCA CGCACCGTGG CAGGCTACGG GCGCTACAGC 1561 GGCAAGATGT ACTTCCAGAC CATGACAGAA GCCGAGTACC AGACAAGCAT CCAGGAGAAG 1621 TTGCCACTCA TCATCGGCTC CTCGGCCGCT GGCCTGGTCT TCCTCATTGC TGTGGTTGTC 1681 ATCGCCATCG TGTGTAACAG AAGACGGGGG TTTGAGCGTG CTGACTCGGA GTACACGGAC 1741 AAGCTGCAAC ACTACACCAG TGGCCACATG ACCCCAGGCA TGAAGATCTA CATCGATCCT 1801 TTCACCTACG AGGACCCCAA CGAGGCAGTG CGGGAGTTTG CCAAGGAAAT TGACATCTCC 1861 TGTGTCAAAA TTGAGCAGGT GATCGGAGCA GGGGAGTTTG GCGAGGTCTG CAGTGGCCAC 1921 CTGAAGCTGC CAGGCAAGAG AGAGATCTTT GTGGCCATCA AGACGCTCAA GTCGGGCTAC 1981 ACGGAGAAGC AGCGCCGGGA CTTCCTGAGC GAAGCCTCCA TCATGGGCCA GTTCGACCAT 2041 CCCAACGTCA TCCACCTGGA GGGTGTCGTG ACCAAGAGCA CACCTGTGAT GATCATCACC 2101 GAGTTCATGG AGAATGGCTC CCTGGACTCC TTTCTCCGGC AAAACGATGG GCAGTTCACA 2161 GTCATCCAGC TGGTGGGCAT GCTTCGGGGC ATCGCAGCTG GCATGAAGTA CCTGGCAGAC 2221 ATGAACTATG TTCACCGTGA CCTGGCTGCC CGCAACATCC TCGTCAACAG CAACCTGGTC 2281 TGCAAGGTGT CGGACTTTGG GCTCTCACGC TTTCTAGAGG ACGATACCTC AGACCCCACC 2341 TACACCAGTG CCCTGGGCGG AAAGATCCCC ATCCGCTGGA CAGCCCCGGA AGCCATCCAG 2401 TACCGGAAGT TCACCTCGGC CAGTGATGTG TGGAGCTACG GCATTGTCAT GTGGGAGGTG 2461 ATGTCCTATG GGGAGCGGCC CTACTGGGAC ATGACCAACC AGGATGTAAT CAATGCCATT 2521 GAGCAGGACT ATCGGCTGCC ACCGCCCATG GACTGCCCGA GCGCCCTGCA CCAACTCATG 2581 CTGGACTGTT GGCAGAAGGA CCGCAACCAC CGGCCCAAGT TCGGCCAAAT TGTCAACACG 2641 CTAGACAAGA TGATCCGCAA TCCCAACAGC CTCAAAGCCA TGGCGCCCCT CTCCTCTGGC 2701 ATCAACCTGC CGCTGCTGGA CCGCACGATC CCCGACTACA CCAGCTTTAA CACGGTGGAC 2761 GAGTGGCTGG AGGCCATCAA GATGGGGCAG TACAAGGAGA GCTTCGCCAA TGCCGGCTTC 2821 ACCTCCTTTG ACGTCGTGTC TCAGATGATG ATGGAGGACA TTCTCCGGGT TGGGGTCACT 2881 TTGGCTGGCC ACCAGAAAAA AATCCTGAAC AGTATCCAGG TGATGCGGGC GCAGATGAAC 2941 CAGATTCAGT CTGTGGAGGT TTGA
Quality Control: cDNA sequencing results is available upon request.
