EphB2 Protein (His Tag)

EPH Receptor B2 / EphB2 Protein Datasheet

EphB2 Protein Product Information

• EphB2 Synonym: EPHB2 ,CAPB, DRT, EPHT3, ERK, Hek5, MGC87492, PCBC, Tyro5
• Protein Construction: A DNA sequence encoding the extracellular domain ( Met 1 - Leu 543 ) of human EphB2 ( NP_059145.2 ) was fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

EphB2 Protein QC Testing

• Purity: >98 % as determined by SDS-PAGE.
• Bio-activity: Measured by its ability to bind recombinant human EphrinB2 / Fc chimera in a functional ELISA
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 19
• Molecular Mass: The recombinant human EphB2 consists of 536 amino acids and has a calculated molecular mass of 59.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEphB2 is approximately 70-75 kDa due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EphB2 protein

EphB2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EphB2 Protein Description

EphB2, also known as Cek5, Nuk, Erk, Qek5, Tyro5, Sek3, Hek5, and Drt, is a member of the transmembrane Eph receptor tyrosine kinase family (RTKs). Ephrins are divided into the ephrin-A class, anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B class, the transmembrane proteins. The Eph receptor family are divided into two groups based on the similarity of their extracellular domain sequences and affinities for binding ephrin-A and ephrin-B ligands, making up the largest subgroup of the receptor tyrosine kinase (RTK) family. Human EphB2 cDNA encodes a 1055 aa precursor, which includes an 18 aa signal sequence, a 525 aa extracellar domain (ECD) containing a cysteine rich region followed by two fibronectin type I II domains, a 21 aa transmembrane segment, and a 491 aa cytoplamic domain containing the tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif. It is expressed on both sides of the neuronal synapse controlling axon guidance across the embryonic midline, promoting a neuronal fate from neural precursors, and regulating NMDA receptor activity. EphB2 is a receptor for members of the ephrin-B family, and is proposed as a tumor suppressor. It is indicated that aberrant EphB2 expression and activity are implicated in the progression of several cancers.

References

1. Fox, G.M. et al., 1995, Oncogene 10:897-905.
2. Tang, X.X. et al., 1998, Oncogene, 17:521-526.
3. Cowan C.A. et al., 2000, Neuron 26:417-430.
4. Henderson, J.T. et al., 2001, Neuron 32:1041-1056.
5. Katakowski, M. et al., 2005, Neurosci. Lett. 385:204-209.
6. Cramer, K.S. et al., 2006, Dev. Biol. 295:76-89.
7. Pasquale, E.B., 2008, Cell. 133: 38-52.
8. Merlos-Suarez, A. et al., 2008, Curr. Opin. Cell Biol. 20:194-200.