EphB2 / EPHB2 Antibody (Antigen Affinity Purified)

Ephrin Type-B Receptor 2 Antibody ( Antigen Affinity Purified )

EphB2 / EPHB2 Antibody Product Information

• Immunogen: Recombinant human EphB2 protein ( Catalog#10762-H08H )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human EphB2 ( rh EphB2 ; Catalog#10762-H08H ; NP_059145.2 ; Met 1- Leu 543 ). EphB2 specific IgG was purified by human EphB2 affinity chromatography.

EphB2 / EPHB2 Antibody Usage Guide

• Specificity: Human EphB2 / EPHB2
• Direct ELISA: This antibody can be used at 0.5 -1.0 μg/mL with the appropriate secondary reagents to detect human EphB2. The detection limit for human EphB2 is 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

EphB2 / EPHB2 Antibody Background

EphB2, also known as Cek5, Nuk, Erk, Qek5, Tyro5, Sek3, Hek5, and Drt, is a member of the transmembrane Eph receptor tyrosine kinase family (RTKs). Ephrins are divided into the ephrin-A class, anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B class, the transmembrane proteins. The Eph receptor family are divided into two groups based on the similarity of their extracellular domain sequences and affinities for binding ephrin-A and ephrin-B ligands, making up the largest subgroup of the receptor tyrosine kinase (RTK) family. Human EphB2 cDNA encodes a 1055 aa precursor, which includes an 18 aa signal sequence, a 525 aa extracellar domain (ECD) containing a cysteine rich region followed by two fibronectin type I II domains, a 21 aa transmembrane segment, and a 491 aa cytoplamic domain containing the tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif. It is expressed on both sides of the neuronal synapse controlling axon guidance across the embryonic midline, promoting a neuronal fate from neural precursors, and regulating NMDA receptor activity. EphB2 is a receptor for members of the ephrin-B family, and is proposed as a tumor suppressor. It is indicated that aberrant EphB2 expression and activity are implicated in the progression of several cancers. 

References

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