Mouse EPHA7 Gene cDNA Clone / ORF Clone

EPHA7 cDNA Clone Datasheet

EPHA7 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010141.3
• cDNA Size: 2997
• Gene Synonym: Ebk; Ehk3; Mdk1; Cek11; Hek11; Epha7
• Source: Mouse

EPHA7 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Eph receptor A7 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 2391 A/G not causing the amino acid variation.

EPHA7 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EPHA7 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EPHA7 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTTGTTC AAACTCGGTT CCCTTCGTGG ATTATTTTGT GTTACATCTG GCTGCTTGGC
61       TTTGCACACA CGGGGGAGGC GCAGGCTGCG AAGGAAGTAC TATTACTGGA CTCGAAAGCA
121      CAACAAACAG AATTGGAATG GATTTCCTCT CCACCCAGTG GGTGGGAAGA AATTAGTGGT
181      TTGGATGAGA ACTACACTCC GATAAGAACA TACCAGGTGT GCCAGGTCAT GGAGCCCAAC
241      CAGAACAACT GGCTGCGGAC TAACTGGATT TCTAAAGGCA ACGCACAAAG GATTTTTGTA
301      GAATTGAAAT TCACCTTGAG GGATTGTAAT AGTCTTCCCG GAGTCCTGGG AACTTGCAAG
361      GAAACGTTTA ATTTGTACTA TTATGAAACA GACTACGACA CCGGCAGGAA TATACGAGAA
421      AACCTTTATG TTAAAATAGA CACCATTGCT GCAGATGAAA GTTTCACACA AGGTGACCTT
481      GGTGAAAGAA AGATGAAGCT GAACACTGAG GTGAGAGAGA TTGGACCTTT GTCCAAAAAG
541      GGATTCTATC TTGCCTTTCA GGATGTAGGG GCTTGCATAG CATTGGTTTC TGTCAAAGTG
601      TACTACAAGA AGTGCTGGTC CATTGTTGAG AACTTAGCTG TCTTTCCAGA TACAGTGACT
661      GGTTCGGAAT TTTCCTCCTT AGTCGAGGTC CGTGGGACAT GTGTCAGCAG TGCCGAGGAA
721      GAGGCAGAAA ATTCCCCCAG AATGCATTGC AGTGCAGAAG GAGAGTGGCT AGTACCCATT
781      GGAAAATGCA TCTGCAAAGC AGGCTATCAG CAAAAAGGGG ACACTTGCGA ACCCTGTGGC
841      CGCAGGTTCT ACAAATCTTC CTCTCAGGAT CTCCAGTGTT CTCGTTGTCC AACCCACAGC
901      TTCTCTGACC GAGAAGGATC ATCCAGGTGT GAATGTGAAG ATGGGTACTA CAGAGCTCCT
961      TCTGATCCAC CATACGTTGC ATGCACGAGG CCTCCCTCTG CACCACAGAA CCTTATTTTC
1021     AATATCAATC AAACGACTGT AAGTTTGGAA TGGAGTCCTC CGGCTGACAA CGGGGGAAGA
1081     AACGATGTCA CCTACAGAAT ACTGTGTAAG CGGTGCAGTT GGGAACAGGG AGAATGTGTG
1141     CCATGCGGAA GTAACATTGG ATACATGCCC CAGCAGACGG GATTAGAGGA TAACTATGTC
1201     ACTGTCATGG ACCTACTTGC CCATGCAAAT TACACTTTCG AAGTTGAAGC TGTAAATGGA
1261     GTTTCGGACT TAAGCAGATC CCAGAGGCTC TTCGCTGCTG TTAGCATCAC CACCGGTCAA
1321     GCAGCTCCCT CGCAAGTGAG TGGAGTCATG AAGGAGCGAG TACTGCAGCG GAGTGTGCAG
1381     CTTTCCTGGC AGGAGCCGGA GCATCCCAAT GGAGTCATCA CGGAATATGA AATCAAGTAT
1441     TATGAGAAAG ATCAACGGGA AAGGACGTAC TCAACACTCA AAACCAAGTC CACCTCCGCC
1501     TCCATTAATA ATCTGAAACC GGGAACAGTG TACGTCTTTC AGATCCGGGC GGTCACTGCT
1561     GCCGGTTATG GAAACTACAG CCCTAGGCTT GATGTTGCCA CACTTGAGGA AGCTTCAGGT
1621     AAAATGTTTG AAGCGACAGC AGTCTCCAGT GAACAGAATC CTGTCATCAT AATTGCTGTA
1681     GTGGCTGTAG CAGGGACCAT CATCTTGGTG TTCATGGTGT TCGGCTTCAT CATTGGAAGA
1741     AGGCACTGTG GTTATAGCAA GGCTGACCAA GAAGGGGATG AAGAACTCTA CTTTCATTTT
1801     AAATTTCCAG GCACCAAAAC CTACATTGAC CCTGAAACCT ATGAGGACCC AAATAGAGCT
1861     GTCCATCAAT TCGCCAAGGA GCTAGATGCC TCCTGTATTA AAATTGAGCG TGTGATTGGT
1921     GCAGGAGAAT TTGGAGAAGT TTGCAGTGGT CGTTTGAAAC TTCCGGGGAA GAGAGATGTT
1981     GCAGTGGCCA TAAAAACCCT GAAAGTTGGT TACACAGAAA AGCAAAGGAG GGACTTTTTA
2041     TGCGAAGCAA GCATCATGGG GCAATTTGAC CACCCAAATG TCGTCCATTT GGAAGGGGTT
2101     GTTACAAGAG GGAAGCCTGT CATGATTGTG ATAGAGTTCA TGGAGAATGG AGCCCTGGAT
2161     GCATTTCTCA GGAAACACGA TGGGCAGTTT ACAGTCATTC AGTTGGTAGG AATGTTGAGA
2221     GGTATTGCCG CTGGGATGCG ATACTTGGCT GATATGGGAT ACGTTCACAG GGACCTTGCA
2281     GCGCGCAACA TCCTTGTCAA CAGCAATCTT GTTTGTAAAG TGTCAGATTT TGGCCTTTCC
2341     CGGGTTATAG AGGATGATCC CGAAGCTGTC TACACCACGA CTGGTGGAAA GATTCCAGTA
2401     AGGTGGACTG CACCGGAAGC CATTCAATAC CGGAAGTTCA CCTCAGCCAG CGATGTGTGG
2461     AGCTATGGGA TTGTCATGTG GGAAGTGATG TCTTATGGAG AAAGACCTTA CTGGGACATG
2521     TCAAATCAAG ATGTCATTAA AGCGATAGAA GAAGGTTATC GTTTGCCGGC GCCCATGGAT
2581     TGCCCAGCTG GTCTTCACCA GCTAATGCTG GATTGTTGGC AGAAAGATCG GGCGGAAAGG
2641     CCAAAGTTTG AGCAGATAGT CGGAATTCTA GACAAAATGA TTCGAAACCC AAGTAGTCTG
2701     AAAACACCCC TGGGAACTTG TAGTAGACCC TTAAGCCCTC TTCTGGACCA GAGCACTCCT
2761     GACTTCACTG CCTTCTGTTC AGTTGGAGAA TGGTTGCAAG CTATTAAAAT GGAAAGGTAT
2821     AAGGACAACT TCACAGCAGC GGGTTACAAC TCACTCGAGT CAGTGGCCAG GATGACTATC
2881     GATGATGTGA TGAGTTTAGG GATCACACTG GTTGGCCATC AAAAGAAGAT CATGAGCAGC
2941     ATCCAGACTA TGCGGGCACA AATGTTGCAT TTACACGGAA CAGGCATCCA AGTGTGA

 

Quality Control:cDNA sequencing results is available upon request.