Mouse EPHA7 / EHK-3 Protein (His Tag)

Mouse Ephrin Type-A Receptor 7 / EPH Homology Kinase 3 Protein

EPHA7 / EHK3 Protein Product Information

• Synonym: RP23-33D17.1, Cek11, Ebk, Ehk3, Hek11, Mdk1
• Protein Construction: A DNA sequence encoding the mouse EPHA7 isoform 1 ( Q61772-1 ) extracellular domain ( Met 1 - Ile 556 ) was fused with a polyhistidine tag at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

EPHA7 / EHK3 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA
  Immobilized mouse EphA7 at 2 μg/ml ( 100 μl/well ) can bind mouse EphrinA4 with a linear range of 0.08 - 10 ng/ml
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Lys 30
• Molecular Mass: The secreted recombinant mouse EPHA7 consists of 537 amino acids and has a predicted molecular mass of 60.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEPHA7 is approximately 70 kDa due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EPHA7 protein

EPHA7 / EHK3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EPHA7 / EHK3 Protein Description

Mouse ephrin type-A receptor 7, also known as EPH homology kinase 3, EPH-like kinase 11, EPHA7 and EHK3, is a single-pass type I  membrane protein which Belongs to the protein kinase superfamily, Tyr protein kinase family and Ephrin receptor subfamily. EPHA7 is widely expressed. It contains two fibronectin type-III domains, one protein kinase domain and one SAM (sterile alpha motif) domain. EPHA7 is a receptor for members of the ephrin-A family. It binds to ephrin-A1, ephrin-A2, ephrin-A3, ephrin-A4 and ephrin-A5. EPHA7 is a member of the EPHA family of receptor kinases, among which several members are known to be involved in human lung carcinogenesis. EPHA7 is expressed at a substantial level in most human lung cancers and the secreted form of EPHA7 mRNA was found in a fraction of primary lung cancer tissues, lung cancer cell lines, and immortalized bronchogenic epithelial cell lines. The high expression of EphA7 protein may play an important role in the malignancy transformation, invasion progression and metastasis of primary hepatocellular carcinoma. EPHA7 warrants further investigation to determine its possible involvement in smoking related lung carcinogenesis.

References

1. Wang, J. et al., 2005, Oncogene. 24 (36):5637-47.
2. García-Calero,E. et al., 2006, Neuroscience. 141 (4):1887-97.
3. Figueroa,JD. et al., 2006, Neurosci Res. 84 (7):1438-51.
4. Wang,J. et al., 2007, Hum Pathol. 38 (11):1649-56.
5. Tsuboi, M. et al., 2010, Int J Oncol. 36 (3):635-40.