Mouse EphA6/EHK-2 Gene cDNA Clone / ORF Clone

EPHA6 cDNA Clone Datasheet

EPHA6 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007938.2
• cDNA Size: 3108
• Gene Synonym: Ehk2; Hek12; m-ehk2; Epha6
• Source: Mouse

EPHA6 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Eph receptor A6 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations:2178 G/A and 2706 G/A not causing the amino acid variation.

EPHA6 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EPHA6 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EPHA6 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGGGCT GCGAAGTCCG GGAATTTCTT TTGCAATTTG GTTTCTTCTT GCCCCTGCTA
61       ACGGCTTGGA CCGGCGACTG CAGTCACGTC TCCAACCAAG TTGTGTTGCT TGATACAACC
121      ACAGTGATGG GAGAACTAGG ATGGAAAACA TATCCATTAA ATGGGTGGGA TGCCATTACT
181      GAAATGGATG AACACAATAG GCCCATACAT ACATACCAGG TATGCAATGT CATGGAACCA
241      AACCAGAACA ACTGGCTTCG TACTAACTGG ATCTCTCGTG ATGCTGCACA GAAAATTTAT
301      GTGGAAATGA AGTTCACACT GAGGGATTGT AACAGCATCC CATGGGTCTT GGGGACTTGT
361      AAAGAAACCT TTAACCTGTA TTATATAGAA TCCGATGAAT CCCATGGGAC AAAATTCAAG
421      CCAAGCCAAT ATATAAAGAT CGACACAATT GCTGCTGATG AGAGTTTTAC TCAGATGGAT
481      TTGGGTGACC GCATCCTTAA ACTCAACACT GAAATCCGTG AGGTGGGGCC TATAGAAAGG
541      AAAGGATTTT ATTTGGCTTT TCAAGATATT GGAGCATGCA TCGCTCTGGT CTCAGTCCGA
601      GTTTTCTACA AAAAATGCCC CTTCACCGTG CGGAACTTGG CTATGTTTCC TGATACCATC
661      CCAAGGGTTG ATTCTTCCTC TTTGGTTGAA GTGCGGGGCT CATGTGTAAA GAGTGCTGAG
721      GAGCGAGATA CTCCTAAACT CTACTGTGGA GCTGATGGGG ATTGGCTTGT TCCTCTTGGA
781      AGGTGTATCT GCAGTACAGG GTATGAAGAA ATCGAGGGTT CTTGCCATGC TTGCAGACCA
841      GGATTCTACA AAGCATTTGC TGGGAACACA AAATGTTCCA AATGCCCTCC ACACAGCTCA
901      ACCTACGTGG AAGCAACGTC AGTCTGTCAT TGTGAAAAGG GTTACTTCCG GGCAGAAAAA
961      GACCCACCTT CTATGGCATG CACTAGACCA CCTTCAGCTC CTAGAAATGT GGCTTTTAAC
1021     ATCAATGAAA CAGCCCTTAT TTTGGAATGG AGCCCACCCA GTGACACAGG AGGGAGAAAA
1081     GATCTCACAT ACAGTGTAAT CTGTAAGAAA TGTGGTTTAG ACACTACCCA GTGTGAGGAC
1141     TGTGGTGGAG GACTCCGCTT CATCCCAAGA CACACTGGAC TGATCAACAA TTCTGTGGTA
1201     GTACTGGACT TTGTGTCTCA CGTCAATTAT ACCTTTGAAA TAGAAGCCAT GAATGGAGTT
1261     TCTGAGTTGA GCATCTCTCC CAAGCCATTC ACAGCTATTA CAGTGACTAC AGATCACGAT
1321     GCACCTTCTC TGATTGGTAT GATGAGAAAG GACTGGGCAT CTCAGAACAG CCTTGCTCTA
1381     TCGTGGCAAG CACCTGCATT TTCCAATGGA GCTATTCTGG ACTATGAGAT CAAGTACTAC
1441     GAGAAAGAGC ATGAGCAGCT CACCTATTCC TCCACGAGGT CCAAGGCCCC AAGCGTCATC
1501     GTCACGGGCC TCAAGCCCGC CACCACGTAC ATATTTCACA TCCGAGTGAG GACGGCGACA
1561     GGCTACAGTG GCTACAGTCA GAAGTTTGAA TTTGAAACAG GAGATGAAAC TTCTGACATG
1621     GCGGCAGAAC AAGGGCAGAT TCTGGTCATA GCCACTGCAG CCGTCGGGGG ATTCACTCTC
1681     TTAGTCATCC TCACCCTGTT CTTCCTCATC ACTGGGAGGT GTCAATGGTA CATAAAGGCC
1741     AAAATGAAGT CAGAAGAGAA GAGAAGAACT CACTTACAGA ACGGCCACCT GCGCTTCCCG
1801     GGAATTAAAA CATACATTGA TCCAGACACC TATGAAGACC CATCCCTAGC AGTCCACGAA
1861     TTTGCAAAAG AGATTGATCC TTCAAGAATT CGCATTGAGA GAGTGATTGG AGCAGGTGAA
1921     TTTGGAGAAG TCTGCAGTGG GCGTTTGAAG ACACCAGGGA AAAGGGAGAT CCCAGTTGCA
1981     ATTAAAACAT TGAAAGGTGG CCACATGGAC CGACAAAGAA GAGATTTTCT AAGAGAAGCT
2041     AGCATCATGG GTCAGTTTGA CCACCCAAAC ATCATTCGCC TAGAAGGTGT TGTCACTAAA
2101     AGATCCTTCC CGGCGATTGG GGTGGAAGCC TTCTGCCCCA GCTTCCTAAG GGCTGGGTTT
2161     TTAAATGGCA TCCAAGCACC ACATCCAGTG ACTGCAGGAG GCTCTCTGCC CCCCAGGATT
2221     CCTGCAGGTC GGCCAGTAAT GATCGTAGTA GAGTATATGG AGAATGGATC ACTGGACTCC
2281     TTTTTGCGGA AGCATGATGG CCACTTCACC GTCATCCAGT TGGTCGGCAT GCTGAGGGGC
2341     ATCGCGTCAG GCATGAAGTA TCTTTCTGAC ATGGGATATG TTCATCGAGA TCTTGCCGCG
2401     AGGAACATAC TGGTGAACAG CAACTTAGTA TGCAAGGTCT CTGATTTTGG TCTCTCCCGA
2461     GTGCTGGAAG ATGATCCAGA AGCAGCTTAT ACAACAACGG GAGGGAAAAT CCCTATACGG
2521     TGGACAGCCC CAGAAGCTAT TGCTTACAGA AAGTTCTCCT CAGCCAGTGA CGCGTGGAGC
2581     TACGGGATTG TCATGTGGGA GGTGATGTCC TATGGAGAGA GACCGTACTG GGAAATGTCC
2641     AACCAGGATG TTATCTTGTC CATTGAAGAA GGTTACCGAC TTCCTGCTCC GATGGGCTGC
2701     CCACCATCTC TGCACCAGCT GATGCTCCAC TGCTGGCAGA AGGAGAGAAA CCACAGGCCA
2761     AAATTCACTG ACATCGTCAG CTTCCTTGAC AAACTGATCC GCAACCCCAG TGCCCTTCAC
2821     ACGCTGGTGG AGGACATCCT TGTAATGCCA GAATCCCCTG GTGATGTTCC TGAATATCCA
2881     TTGTTTGTCA CAGTAGGTGA CTGGCTGGAT TCTATAAAGA TGGGGCAATA CAAGAGTAAC
2941     TTCATGGCAG CGGGTTTTAC AACGTTTGAT CTGATTTCAC GAATGAGCAT CGATGATATT
3001     AGGCGAATTG GAGTCATTCT CATTGGACAT CAGAGACGAA TAGTCAGCAG CATACAAACT
3061     TTACGTTTAC ATATGATGCA CATACAGGAA AAAGGATTTC ATGTATGA

 

Quality Control:cDNA sequencing results is available upon request.