Mouse EPHA6 / EHK-2 Protein (Fc Tag)

Mouse Ephrin Type-A Receptor 6 / EPH Homology Kinase 2 Protein

EphA6 / Ehk-2 Protein Product Information

• Synonym: Epha6, Ehk2, Hek12, m-ehk2
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse EphA6 ( NP_031964.2 ) ( Met 1 - Gln 546 ) was fused with the Fc region of human IgG1 at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

EphA6 / Ehk-2 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA
  Immobilized mouse EphrinA3 at 1 μg/ml ( 100 μl/well ) can bind mouse EPHA6 / chimera with a linear ranger of 6.25 - 400 ng/ml
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 28
• Molecular Mass: The recombinant mouse EphA6/Fc is a disulfide-linked homodimer. The reduced monomer consists of 760 amino acids and has a predicted molecular mass of 85 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm EphA6 / Fc monomer is approximately 110 kDa due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EphA6 protein

EphA6 / Ehk-2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EphA6 / Ehk-2 Protein Description

Mouse Ephrin type-A receptor 6, also known as EPH homology kinase 2, Epha6, and Ehk-2, is a single-pass type I  membrane protein which belongs to the protein kinase superfamily and Ephrin receptor subfamily. It contains two fibronectin type-III domains, one protein kinase domain, and one SAM (sterile alpha motif) domain. EphA6, in line with a number of other Eph receptors and their ephrin ligands, is involved in neural circuits underlying aspects of learning and memory. EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4 and EPHB6 are EPH family receptors for Ephrin family ligands. Ephrin/EPH signaling pathway networks with the WNT signaling pathway during embryogenesis, tissue regeneration, and carcinogenesis. In molecular biology, ephrins and Eph receptors are components of cell signaling pathways involved in animal development, and implicated in some cancers. Eph receptors are classified as receptor tyrosine kinases (RTKs), and form the largest sub-family of RTKs. Almost all Eph receptors are expressed during various well defined stages of development in assorted locations and concentrations. 

References

1. Hafner, C. et al., 2004, Clin Chem. 50 (3): 490-9.
2. Katoh,M. et al., 2006, Int J Oncol. 28 (5): 1243-7.
3. Shaut, CA. et al., 2007, Dev Dyn. 236 (4): 951-60.
4. Savelieva, KV. et al., 2008, Neurosci Lett. 438 (2): 205-9.