Mouse EPHA4 Gene cDNA Clone / ORF Clone

EPHA4 cDNA Clone Datasheet

EPHA4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007936.3
• cDNA Size: 2961
• Gene Synonym: rb; Sek; Cek8; Hek8; Sek1; Tyro1; AI385584; 2900005C20Rik; Epha4
• Source: Mouse

EPHA4 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Eph receptor A4 DNA.
• Vector: Cloning Vector: pMD18-T Simple Vector.

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

EPHA4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EPHA4 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EPHA4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGGGA TTTTCTATTT CATCCTCTTT TCGTTTCTCT TTGGAATTTG CGACGCTGTC
61       ACCGGTTCTA GGGTATACCC CGCGAATGAA GTTACTTTAT TGGATTCCAG ATCTGTTCAG
121      GGAGAGCTTG GGTGGATAGC AAGCCCTCTG GAAGGAGGGT GGGAGGAAGT GAGCATTATG
181      GATGAGAAAA ATACACCGAT CCGAACCTAC CAGGTGTGCA ACGTGATGGA AGCCAGCCAG
241      AACAACTGGC TGCGAACTGA CTGGATCACC CGCGAAGGGG CGCAGAGGGT GTACATTGAA
301      ATTAAGTTCA CTCTAAGGGA CTGCAACAGT CTTCCGGGCG TCATGGGGAC TTGCAAGGAG
361      ACGTTTAACC TCTACTACTA TGAGTCGGAC AACGACAAGG AGCGCTTCAT CAGAGAGAGC
421      CAGTTTGGCA AGATCGACAC CATCGCAGCT GATGAGAGCT TCACGCAGGT GGACATTGGT
481      GACCGAATCA TGAAACTCAA CACTGAGATC CGGGATGTAG GGCCACTGAG CAAGAAAGGG
541      TTTTACCTGG CTTTTCAGGA TGTGGGTGCT TGCATCGCCC TGGTGTCTGT CCGTGTGTTC
601      TACAAGAAGT GTCCACTCAC CGTTCGAAAT CTAGCCCAGT TTCCTGATAC CATTACTGGG
661      GCTGATACCT CTTCCCTGGT GGAAGTTCGA GGCTCCTGTG TCAACAACTC AGAAGAGAAG
721      GATGTGCCAA AAATGTACTG TGGGGCAGAT GGTGAATGGC TGGTACCCAT TGGCAACTGC
781      CTATGCAACG CTGGGCACGA GGAGCAGAAT GGTGAATGCC AAGCTTGCAA AATTGGATAT
841      TACAAAGCTC TTTCCACGGA CGCCTCCTGT GCCAAGTGTC CACCGCACAG CTACTCTGTC
901      TGGGAAGGAG CCACGTCCTG CACCTGTGAC CGAGGCTTTT TCAGAGCTGA CAACGATGCA
961      GCCTCCATGC CCTGCACCCG CCCACCATCT GCTCCCCTCA ACTTGATTTC TAATGTCAAC
1021     GAGACATCGG TGAACTTGGA ATGGAGCAGC CCTCAGAACA CAGGTGGCCG TCAGGACATT
1081     TCTTACAACG TGGTCTGCAA GAAATGTGGA GCTGGTGATC CCAGCAAGTG CCGGCCCTGT
1141     GGAAGTGGAG TCCACTACAC ACCACAGCAG AACGGACTCA AGACGACCAG AGTCTCCATC
1201     ACTGACCTCC TAGCACACAC CAATTACACG TTTGAGATCT GGGCAGTGAA TGGAGTGTCT
1261     AAGTATAACC CTAGCCCGGA CCAGTCCGTG TCTGTCACAG TGACAACCAA CCAAGCAGCA
1321     CCATCATCCA TTGCTTTGGT CCAGGCTAAA GAAGTTACAA GATATAGCGT AGCTCTGGCT
1381     TGGCTGGAAC CAGATCGACC AAATGGAGTC ATCTTAGAAT ATGAAGTCAA ATATTATGAA
1441     AAGGATCAGA ATGAACGCAG CTATCGCATA GTCCGGACAG CTGCCAGGAA CACAGATATC
1501     AAAGGCCTGA ACCCTCTGAC TTCCTATGTT TTTCACGTGC GAGCCAGGAC CGCTGCTGGC
1561     TACGGAGACT TCAGCGAGCC CCTGGAAGTC ACTACTAATA CAGTGCCTTC CCGCATCATT
1621     GGCGATGGAG CCAACTCCAC TGTCCTGCTG GTCTCCGTCT CTGGCAGTGT GGTCCTCGTG
1681     GTCATTCTCA TTGCAGCTTT TGTGATCAGC CGAAGACGGA GTAAGTACAG CAAAGCGAAA
1741     CAAGAAGCAG ATGAAGAGAA ACATTTGAAT CAAGGTGTTA GAACTTATGT GGATCCCTTT
1801     ACATACGAAG ACCCCAACCA GGCAGTTCGA GAATTTGCCA AAGAAATCGA TGCATCCTGC
1861     ATTAAAATTG AAAAGGTCAT AGGAGTTGGT GAATTTGGAG AGGTCTGCAG TGGGCGTCTC
1921     AAAGTGCCCG GCAAGAGAGA GATCTGTGTG GCCATCAAGA CTCTGAAAGC TGGCTATACT
1981     GACAAGCAGA GGAGAGACTT CCTGAGTGAG GCCAGCATCA TGGGACAGTT TGACCACCCA
2041     AACATAATCC ACCTGGAAGG CGTTGTCACC AAATGTAAAC CGGTAATGAT CATAACGGAG
2101     TACATGGAGA ACGGCTCCTT GGATGCTTTC CTCAGGAAGA ATGATGGCCG CTTTACAGTC
2161     ATTCAGCTGG TGGGCATGCT CCGGGGCATT GGCTCGGGGA TGAAGTACTT ATCTGATATG
2221     AGCTATGTGC ATCGAGACCT GGCTGCCAGG AACATCCTGG TAAACAGCAA CTTGGTCTGC
2281     AAGGTGTCTG ACTTTGGCAT GTCCAGGGTG CTGGAGGATG ACCCCGAAGC AGCCTACACT
2341     ACCAGGGGTG GCAAGATTCC TATCCGGTGG ACTGCGCCAG AAGCAATTGC GTATCGTAAA
2401     TTTACCTCAG CCAGTGATGT CTGGAGCTAC GGAATCGTTA TGTGGGAAGT GATGTCATAT
2461     GGAGAGAGAC CGTACTGGGA TATGTCCAAT CAAGATGTGA TCAAAGCCAT CGAGGAAGGG
2521     TACCGGCTAC CCCCTCCAAT GGACTGCCCC ATTGCCCTCC ATCAGTTAAT GCTGGACTGC
2581     TGGCAGAAAG AGAGAAGTGA CAGGCCTAAA TTTGGGCAGA TCGTCAACAT GCTGGACAAA
2641     CTCATCCGCA ATCCCAACAG CCTGAAGAGG ACAGGGTCGG AGAGTTCCAG ACCAAACACA
2701     GCCTTGTTAG ATCCCAGCTC CCCTGAATTC TCTGCTGTAG TATCAGTGGG CGACTGGCTG
2761     CAGGCCATCA AAATGGACCG GTATAAGGAC AACTTCACGG CCGCCGGGTA CACGACACTA
2821     GAAGCCGTGG TTCACATGAG CCAGGACGAC CTGGCGAGAA TTGGCATCAC CGCCATCACG
2881     CACCAGAATA AGATTTTGAG CAGCGTCCAG GCGATGCGAA CCCAGATGCA GCAGATGCAT
2941     GGCAGGATGG TTCCTGTCTG A

 

Quality Control:cDNA sequencing results is available upon request.