EPHA4 / HEK8 / TYRO1 Antibody (Antigen Affinity Purified)

Ephrin Type-A Receptor 4 Antibody ( Antigen Affinity Purified )

EPHA4 / HEK8 / TYRO1 Antibody Product Information

• Immunogen: Recombinant human EPHA4 protein ( Catalog#11314-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human EPHA4 ( rh EPHA4 ; Catalog#11314-H08H ; NP_031962.2 ; Met 1 - Thr 547 ). EPHA4 specific IgG was purified by human EPHA4 affinity chromatography

EPHA4 / HEK8 / TYRO1 Antibody Usage Guide

• Specificity: Human EPHA4 / HEK8 / TYRO1
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human EPHA4 in WB. Using a DAB detection system, the detection limit for human EPHA4 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human EPHA4. The detection limit for Human EPHA4 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

EPHA4 / HEK8 / TYRO1 Antibody Background

Ephrin type-A receptor 4, also known as Tyrosine-protein kinase receptor SEK, Tyrosine-protein kinase TYRO1, EPH-like kinase 8, and EPHA4, is a single-pass type I  membrane protein which belongs to the protein kinase superfamily and ephrin receptor subfamily. EPHA4 contains two fibronectin type-III domains, one protein kinase domain and one SAM (sterile alpha motif) domain. EPHA4 is a receptor for members of the ephrin-A family. EPHA4 binds to ephrin-A1, ephrin-A4 and ephrin-A5. It binds more poorly to ephrin-A2 and ephrin-A3. EPHA4 may play a role in a signal transduction process involved in hindbrain pattern formation. 

The ephrins (Eph) and Eph receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosyl- phosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. In molecular biology, ephrins and Eph receptors are components of cell signaling pathways involved in animal development, and implicated in some cancers. Eph receptors are classified as receptor tyrosine kinases (RTKs), and form the largest sub-family of RTKs.

References

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