Anti-EpCAM Antibody

Cat: 50591-R002
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Anti-EpCAM Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-EpCAM Antibody
Validated applications
WB,ELISA,ELISA(Det),IHC-P
Species reactivity
Reacts with: Mouse
Specificity
Mouse EpCAM
No cross-reactivity in ELISA with Human EPCAM
Immunogen
Recombinant Mouse EpCAM / TROP-1 / TACSTD1 protein (Catalog#50591-M08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse EpCAM / TROP-1 / TACSTD1 (rM EpCAM / TROP-1 / TACSTD1; Catalog#50591-M08H; NP_032558.2; Met 1-Thr 266).
Source
Monoclonal Rabbit IgG Clone #002
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-EpCAM Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IHC-P 1:500-1:2000  
ELISA(Det) 1:5000-1:50000 This antibody will detect Mouse EpCAM / TROP-1 / TACSTD1 in ELISA pair set (Catalog: # SEK50591). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 50591-R052).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-EpCAM Antibody (Rabbit Monoclonal antibody) Images
Mouse EpCAM/TROP-1/TACSTD1 Immunohistochemistry(IHC) 3303
Immunochemical staining of mouse EpCAM in mouse uterus with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Mouse EpCAM/TROP-1/TACSTD1 Immunohistochemistry(IHC) 3306
Immunochemical staining of mouse EpCAM in mouse stomach with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Mouse EpCAM/TROP-1/TACSTD1 Immunohistochemistry(IHC) 3308
Immunochemical staining of mouse EpCAM in mouse intestine with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Mouse EpCAM/TROP-1/TACSTD1 Western blot (WB) 17508

Anti-EPCAM rabbit monoclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:35 kDa

Observed band size:38 kDa

Anti-EpCAM Antibody Alternative Names
Anti-CD326 Antibody;Anti-EGP Antibody;Anti-EGP-2 Antibody;Anti-Egp314 Antibody;Anti-Ep-CAM Antibody;Anti-EpCAM1 Antibody;Anti-GA733-2 Antibody;Anti-gp40 Antibody;Anti-Ly74 Antibody;Anti-Tacsd1 Antibody;Anti-Tacstd1 Antibody;Anti-TROP1 Antibody
EpCAM Background Information

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â… transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
epithelial cell adhesion molecule
References
  • Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
  • Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
  • Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
  • Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-EpCAM Antibody Rabbit Polyclonal ELISA 90299-RP01
    Anti-EpCAM Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 90299-T60
    Anti-EpCAM Antibody Rabbit Polyclonal ELISA,IHC-P 80306-T24
    Anti-EpCAM Antibody Rabbit Polyclonal ELISA 50591-RP01
    Anti-EpCAM Antibody Rabbit Monoclonal ELISA(Cap) 50591-R052
    Anti-EpCAM Antibody Rabbit Polyclonal ELISA,IHC-P 50591-RP02
    Anti-EpCAM Antibody Rabbit Monoclonal IHC-P 50591-R065
    Anti-EpCAM Antibody (PE) Rabbit Monoclonal FCM 10694-R028-P
    Anti-EpCAM Antibody Rabbit Polyclonal WB,ELISA 10694-RP02
    Anti-EpCAM Antibody Rabbit Monoclonal WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IF,IP 10694-R028
    Anti-EpCAM Antibody (APC) Rabbit Monoclonal FCM 10694-R028-A
    Anti-EpCAM Antibody (APC) Mouse Monoclonal FCM 10694-MM06-A
    Anti-EpCAM Antibody Rabbit Polyclonal WB,ELISA,IP 10694-T52
    Anti-EpCAM Antibody Rabbit Polyclonal ELISA 10694-RP01
    Anti-EpCAM Antibody (FITC) Mouse Monoclonal FCM 10694-MM06-F
    Anti-EpCAM Antibody Mouse Monoclonal FCM 10694-MM06
    Anti-EpCAM Antibody (PE) Mouse Monoclonal FCM 10694-MM06-P
    Anti-EpCAM Antibody (FITC) Rabbit Monoclonal FCM 10694-R028-F
    Anti-EpCAM Antibody Mouse Monoclonal ELISA,ELISA(Cap) 10694-MM07
    Anti-EpCAM Antibody Mouse Monoclonal WB,IP 10694-MM05
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