Anti-EpCAM Antibody
Cat: 10694-R028
Anti-EpCAM Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-EpCAM Antibody
Validated applications
WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human EpCAM
No cross-reactivity in ELISA with
Human CD146 / MCAM
Human CD171 / N-CAML1 / L1CAM
Human OBCAM / OPCML
Human TROP2 / TACSTD2
No cross-reactivity in ELISA with
Human CD146 / MCAM
Human CD171 / N-CAML1 / L1CAM
Human OBCAM / OPCML
Human TROP2 / TACSTD2
Immunogen
Recombinant Human EpCAM protein (Catalog#10694-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human EpCAM / TROP1 (rh EpCAM; Catalog#10694-H08H; NP_002345.1; Met 1-Lys 265).
Source
Monoclonal Rabbit IgG Clone #28
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-EpCAM Antibody (Rabbit Monoclonal antibody) Validated Applications
| Application | Dilution |
|---|---|
| WB | 1:500-1:1000 |
| ELISA | 1:25000-1:50000 |
| IHC-P | 1:1000-1:5000 |
| ICC-IF | 1:250-1:5000 |
| FCM | 1:100-1:500 |
| IP | 0.2-1 μL/mg of lysate |
| ELISA(Det) | 1:5000-1:50000 |
Notes
| ELISA(Det)： | This antibody will detect Human EpCAM / TROP-1 / TACSTD1 in ELISA pair set (Catalog: # SEK10694). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10694-MM07). |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Anti-EpCAM Antibody (Rabbit Monoclonal antibody) Images
Immunofluorescence staining of Human EpCAM in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human EpCAM monoclonal antibody (1:500) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Immunochemical staining of human EpCAM in human mammary gland with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of alveolus epithelium.
Immunochemical staining of human EpCAM in human colon carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of colonic gland epithelium.
Immunochemical staining of human EpCAM in human bladder carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of transitional epithelium.
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Flow cytometric analysis of anti-EpCAM (CD326) reactivity on SKBR3 cells. SKBR3 cells were stained with anti-EpCAM (CD326) Monoclonal Ab (10694-R028), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of viable cells.
Anti-EpCAM rabbit monoclonal antibody at 1:500 dilution
Lane A: MCF-7 Whole Cell Lysate
Lysates/proteins at 30 μg per lane.Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.Performed under reducing conditions.
Predicted band size:34 kDaObserved band size:38 kDa
EpCAM was immunoprecipitated using:
Lane A:0.5 mg MCF-7 Whole Cell Lysate
Lane B:0.5 mg A431 Whole Cell Lysate
Lane C:0.5 mg HepG2 Whole Cell Lysate
Lane D:0.5 mg Caco-2 Whole Cell Lysate
0.5 µL anti-EpCAM rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-EpCAM rabbit monoclonal antibody,at 1:5000 dilution
Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution
Developed using the odssey technique.Performed under reducing conditions.
Predicted band size: 40 kDaObserved band size: 40 kDa
Anti-EpCAM Antibody: Alternative Names
Anti-DIAR5 Antibody; Anti-EGP-2 Antibody; Anti-EGP314 Antibody; Anti-EGP40 Antibody; Anti-ESA Antibody; Anti-HNPCC8 Antibody; Anti-KS1/4 Antibody; Anti-KSA Antibody; Anti-M4S1 Antibody; Anti-MIC18 Antibody; Anti-MK-1 Antibody; Anti-TACSTD1 Antibody; Anti-TROP-1 Antibody; Anti-TROP1 Antibody
EpCAM Background Information
Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
Full Name
epithelial cell adhesion molecule
Research Areas
- Cancer>>Cancer Biomarker
- Stem Cells>>Cancer Stem Cells (CSCs)>>Cancer Stem Cell (CSC) Marker
- Developmental Biology>>Embryogenesis>>Germ Layer Formation>>Ectoderm Marker
- Cancer>>Cancer Types>>Hepatoma
- Immunology>>Cluster of Differentiation (CD)>>Other CD Antigen
- Cancer>>Cancer Types>>Reproductive system cancer
- Stem Cells>>Trophoblast Stem Cells>>Trophoblast Stem Cell Marker
References
- Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
- Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
- Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
- Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
