|IP||0.2-1 μL/mg of lysate|
|ELISA(Det)||1:5000-1:50000||This antibody will detect Human EpCAM / TROP-1 / TACSTD1 in ELISA pair set (Catalog: # SEK10694). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10694-MM07).|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
EpCAM was immunoprecipitated using:
Lane A:0.5 mg MCF-7 Whole Cell Lysate
Lane B:0.5 mg A431 Whole Cell Lysate
Lane C:0.5 mg HepG2 Whole Cell Lysate
Lane D:0.5 mg Caco-2 Whole Cell Lysate0.5 µL anti-EpCAM rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-EpCAM rabbit monoclonal antibody,at 1:5000 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 40 kDa
Observed band size: 40 kDa
Anti-EpCAM rabbit monoclonal antibody at 1:500 dilution
Lane A: MCF-7 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:34 kDa
Observed band size:38 kDa
Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â… transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-EpCAM Antibody (PE)||Rabbit||Monoclonal||FCM||10694-R028-P|
|Anti-EpCAM Antibody (APC)||Rabbit||Monoclonal||FCM||10694-R028-A|
|Anti-EpCAM Antibody (APC)||Mouse||Monoclonal||FCM||10694-MM06-A|
|Anti-EpCAM Antibody (FITC)||Mouse||Monoclonal||FCM||10694-MM06-F|
|Anti-EpCAM Antibody (PE)||Mouse||Monoclonal||FCM||10694-MM06-P|
|Anti-EpCAM Antibody (FITC)||Rabbit||Monoclonal||FCM||10694-R028-F|