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Anti-EpCAM Antibody

Cat: 10694-R028

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Anti-EpCAM Antibody (Rabbit Monoclonal antibody) General Information

Product name

Anti-EpCAM Antibody

Validated applications

WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IF,IP

Species reactivity

Reacts with: Human

Specificity

Human EpCAM
No cross-reactivity in ELISA with Human CD146 / MCAM Human CD171 / N-CAML1 / L1CAM Human OBCAM / OPCML Human TROP2 / TACSTD2

Immunogen

Recombinant Human EpCAM protein (Catalog#10694-H08H)

Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human EpCAM / TROP1 (rh EpCAM; Catalog#10694-H08H; NP_002345.1; Met 1-Lys 265).

Source

Monoclonal Rabbit IgG Clone #28

Purification

Protein A

Formulation

0.2 μm filtered solution in PBS

Conjugate

Unconjugated

Form

Liquid

Shipping

This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-EpCAM Antibody (Rabbit Monoclonal antibody) Validated Applications

Application	Dilution	Notes
WB	1:500-1:1000
ELISA	1:25000-1:50000
IHC-P	1:1000-1:5000
ICC-IF	1:250-1:5000
FCM	1:100-1:500
IP	0.2-1 μL/mg of lysate
ELISA(Det)	1:5000-1:50000	This antibody will detect Human EpCAM / TROP-1 / TACSTD1 in ELISA pair set (Catalog: # SEK10694). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10694-MM07).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-EpCAM Antibody (Rabbit Monoclonal antibody) Images

Immunofluorescence staining of Human EpCAM in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human EpCAM monoclonal antibody (1:500) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.

Immunochemical staining of human EpCAM in human mammary gland with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of alveolus epithelium.

Immunochemical staining of human EpCAM in human colon carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of colonic gland epithelium.

Immunochemical staining of human EpCAM in human bladder carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of transitional epithelium.

High Five cells were infected by Bac-NP (solid line histogram) or Bac-NA (dashed line histogram), the cells were collected at 48 hours postinfection, and the levels of NP were measured by flow cytometry. Cells were fixed and permeabilized according to the manufacturer’s manual (Cat. No. 554714, BD Pharmingen) and subsequently stained with Purified Mouse Anti-H1N1-NP (11675-MM03) antibody for 20 min on ice. Cells were washed twice and incubated with 1 μg of a FITC Goat Anti-Mouse Ig secondary antibody for 20 min on ice. Cells were washed twice and analyzed by flow cytometry.

Flow cytometric analysis of anti-EpCAM (CD326) reactivity on SKBR3 cells. SKBR3 cells were stained with anti-EpCAM (CD326) Monoclonal Ab (10694-R028), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of viable cells.

EpCAM was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

Lane B:0.5 mg A431 Whole Cell Lysate

Lane C:0.5 mg HepG2 Whole Cell Lysate

Lane D:0.5 mg Caco-2 Whole Cell Lysate

0.5 µL anti-EpCAM rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-EpCAM rabbit monoclonal antibody,at 1:5000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 40 kDa

Observed band size: 40 kDa

Anti-EpCAM rabbit monoclonal antibody at 1:500 dilution

Lane A: MCF-7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:34 kDa

Observed band size:38 kDa

Anti-EpCAM Antibody Alternative Names

Anti-DIAR5 Antibody;Anti-EGP-2 Antibody;Anti-EGP314 Antibody;Anti-EGP40 Antibody;Anti-ESA Antibody;Anti-HNPCC8 Antibody;Anti-KS1/4 Antibody;Anti-KSA Antibody;Anti-M4S1 Antibody;Anti-MIC18 Antibody;Anti-MK-1 Antibody;Anti-TACSTD1 Antibody;Anti-TROP-1 Antibody;Anti-TROP1 Antibody

EpCAM Background Information

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â… transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name

epithelial cell adhesion molecule

Research Areas

• Cancer>>Cancer Biomarker
• Stem Cells>>Cancer Stem Cells (CSCs)>>Cancer Stem Cell (CSC) Marker
• Developmental Biology>>Embryogenesis>>Germ Layer Formation>>Ectoderm Marker
• Cancer>>Cancer Types>>Hepatoma
• Immunology>>Cluster of Differentiation (CD)>>Other CD Antigen
• Cancer>>Cancer Types>>Reproductive system cancer
• Stem Cells>>Trophoblast Stem Cells>>Trophoblast Stem Cell Marker

References

Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.

Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.

Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.

Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.

Product Description	Host	Clonality	Application	Catalog# (PDF)
Anti-EpCAM Antibody	Rabbit	Polyclonal	ELISA	90299-RP01
Anti-EpCAM Antibody	Rabbit	Polyclonal	WB,ELISA,IHC-P,IP	90299-T60
Anti-EpCAM Antibody	Rabbit	Polyclonal	ELISA,IHC-P	80306-T24
Anti-EpCAM Antibody	Rabbit	Polyclonal	ELISA	50591-RP01
Anti-EpCAM Antibody	Rabbit	Monoclonal	WB,ELISA,ELISA(Det),IHC-P	50591-R002
Anti-EpCAM Antibody	Rabbit	Monoclonal	ELISA(Cap)	50591-R052
Anti-EpCAM Antibody	Rabbit	Polyclonal	ELISA,IHC-P	50591-RP02
Anti-EpCAM Antibody	Rabbit	Monoclonal	IHC-P	50591-R065
Anti-EpCAM Antibody (PE)	Rabbit	Monoclonal	FCM	10694-R028-P
Anti-EpCAM Antibody	Rabbit	Polyclonal	WB,ELISA	10694-RP02
Anti-EpCAM Antibody (APC)	Rabbit	Monoclonal	FCM	10694-R028-A
Anti-EpCAM Antibody (APC)	Mouse	Monoclonal	FCM	10694-MM06-A
Anti-EpCAM Antibody	Rabbit	Polyclonal	WB,ELISA,IP	10694-T52
Anti-EpCAM Antibody	Rabbit	Polyclonal	ELISA	10694-RP01
Anti-EpCAM Antibody (FITC)	Mouse	Monoclonal	FCM	10694-MM06-F
Anti-EpCAM Antibody	Mouse	Monoclonal	FCM	10694-MM06
Anti-EpCAM Antibody (PE)	Mouse	Monoclonal	FCM	10694-MM06-P
Anti-EpCAM Antibody (FITC)	Rabbit	Monoclonal	FCM	10694-R028-F
Anti-EpCAM Antibody	Mouse	Monoclonal	ELISA,ELISA(Cap)	10694-MM07
Anti-EpCAM Antibody	Mouse	Monoclonal	WB,IP	10694-MM05