|Recombinant E.coli Enoyl-ACP Reductase / FABI protein (Catalog#30001-E07E)|
|0.2 μm filtered solution in PBS|
|Produced in rabbits immunized with purified, recombinant E.coli Enoyl-ACP Reductase / FABI (rE.coli Enoyl-ACP Reductase / FABI; Catalog#30001-E07E; NP_287908.1; Gly 2-Lys 262). Enoyl-ACP Reductase / FABI specific IgG was purified by E.coli Enoyl-ACP Reductase / FABI affinity chromatography.|
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect E.Coli FABI. The detection limit for E.Coli FABI is approximately 0.00975 ng/well.
Enoyl-ACP reductase, also known as NADH-dependent enoyl-ACP reductase and FABI, is a cell inner membrane and peripheral membrane protein which belongs to the short-chain dehydrogenases/reductases (SDR) family and FabI subfamily. Microorganisms produce many kinds of antibiotics which function in an antagonistic capacity in nature where they have much competition. Bacterial FAS provides essential fatty acids for use in the assembly of key cellular components. Among them, FABI is an enoyl-ACP reductase which catalyzes the final and rate-limiting step of bacterial FAS. The antibiotic diazaborine interferes with the activity by binding to the protein. FABI is a potential target for selective antibacterial action, because it shows low overall sequence homology with mammalian enzymes. Various compounds have been reported as inhibitors of bacterial FabI-inhibitory compounds.