Endoglin / CD105 / ENG Antibody

Endoglin / CD105 / ENG Antibody Datasheet

Endoglin / CD105 / ENG Antibody Product Information

• Immunogen: Recombinant Mouse Endoglin / CD105 / ENG Protein (Catalog#50407-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 001
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Mouse Endoglin / CD105 / ENG (rM Endoglin / CD105 / ENG; Catalog#50407-M08H; NP_031958.2; Met 1 -Gly 580 ).

Endoglin / CD105 / ENG Antibody Usage Guide

• Specificity: Mouse Endoglin / CD105 / ENG
• No cross-reactivity in ELISA with

  Human CD105

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse ENG in WB. Using a DAB detection system, the detection limit for Mouse ENG is approximately 0.5 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse ENG. The detection limit for Mouse ENG is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Endoglin / CD105 / ENG Antibody Background

Mouse endoglin, also known as CD105, is a type I  homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Endoglin contains an RGD tripeptide, a key recognition structure in cellular adhesion, and suggests a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type I I receptor (TβRII) and activates the downstream signal pathway. In addition, ALK-1 is also a receptor kinase in human umbilical vein endothelial cells for endoglin threonine phosphorylation, and mutations in either result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful marker of neovascularization, and associated with several solid tumor types.

References

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