Anti-Endoglin / CD105 Antibody

Cat: 50407-R001
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All Endoglin / CD105 Reagents
Anti-Endoglin / CD105 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Endoglin / CD105 Antibody
Validated applications
WB,ELISA,FCM
Species reactivity
Reacts with: Mouse
Specificity
Mouse Endoglin / CD105
No cross-reactivity in ELISA with Human CD105
Immunogen
Recombinant Mouse Endoglin / CD105 / ENG Protein (Catalog#50407-M08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Endoglin / CD105 / ENG (rM Endoglin / CD105 / ENG; Catalog#50407-M08H; NP_031958.2; Met 1-Gly 580).
Source
Monoclonal Rabbit IgG Clone #001
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Endoglin / CD105 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
FCM 1:100-1:500  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Endoglin / CD105 Antibody (Rabbit Monoclonal antibody) Images
Mouse Endoglin/CD105  Flow Cytometry (FC) 4954
Flow cytometric analysis of Mouse ENG(CD105) expression on bEnd.3 cells. Cells were stained with purified anti-Mouse ENG(CD105), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Mouse Endoglin/CD105  Western blot (WB) 13672

Anti-ENG rabbit monoclonal antibody at 1:500 dilution

Lane A: MCF7 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:70 kDa

Observed band size:45 kDa

Anti-Endoglin / CD105 Antibody Alternative Names
Anti-AI528660 Antibody;Anti-AI662476 Antibody;Anti-CD105 Antibody;Anti-Endo Antibody;Anti-S-endoglin Antibody
Endoglin / CD105 Background Information

Endoglin, also known as CD105, is a type I  homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type I I receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.

Full Name
endoglin
References
  • Bellon T., et al.,(1993), Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur. J. Immunol. 23:2340-2345.
  • Humphray S.J., et al., (2004), DNA sequence and analysis of human chromosome 9.Nature 429:369-374.
  • Gougos A., et al.,(1990), Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells.J. Biol. Chem. 265:8361-8364.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Endoglin / CD105 Antibody Rabbit Monoclonal ELISA(Det) 50407-R028
    Anti-Endoglin / CD105 Antibody (FITC) Rabbit Monoclonal FCM 50407-R001-F
    Anti-Endoglin / CD105 Antibody Rabbit Monoclonal ELISA(Cap) 50407-R029
    Anti-Endoglin / CD105 Antibody Rabbit Polyclonal ELISA,IHC-P 50407-RP02
    Anti-Endoglin / CD105 Antibody (APC) Rabbit Monoclonal FCM 50407-R001-A
    Anti-Endoglin / CD105 Antibody Rabbit Polyclonal ELISA 50407-RP01
    Anti-Endoglin / CD105 Antibody (PE) Rabbit Monoclonal FCM 50407-R001-P
    Anti-Endoglin / CD105 Antibody (PE) Mouse Monoclonal FCM 10149-MM13-P
    Anti-Endoglin / CD105 Antibody (FITC) Mouse Monoclonal FCM 10149-MM13-F
    Anti-Endoglin / CD105 Antibody (FITC) Rabbit Monoclonal FCM 10149-R103-F
    Anti-Endoglin / CD105 Antibody Rabbit Monoclonal FCM,ICC/IF,IF 10149-R103
    Anti-Endoglin / CD105 Antibody Mouse Monoclonal ELISA 10149-MM12
    Anti-Endoglin / CD105 Antibody (FITC) Mouse Monoclonal FCM 10149-MM11-F
    Anti-Endoglin / CD105 Antibody (PE) Rabbit Monoclonal FCM 10149-R103-P
    Anti-Endoglin / CD105 Antibody (PerCP) Rabbit Monoclonal FCM 10149-R103-C
    Anti-Endoglin / CD105 Antibody Rabbit Polyclonal ELISA,IHC-P,ICC/IF,IF 10149-T26
    Anti-Endoglin / CD105 Antibody Mouse Monoclonal ELISA(Cap) 10149-MM05
    Anti-Endoglin / CD105 Antibody Rabbit Polyclonal WB,ELISA 10149-RP02
    Anti-Endoglin / CD105 Antibody Rabbit Polyclonal IHC-P 100088-RP02
    Anti-Endoglin / CD105 Antibody Rabbit Polyclonal ELISA 10149-RP01
    Anti-Endoglin / CD105 Antibody Rabbit Monoclonal ELISA 10149-R001
    Anti-Endoglin / CD105 Antibody Mouse Monoclonal ELISA,ELISA(Det) 10149-MM11
    Anti-Endoglin / CD105 Antibody (APC) Rabbit Monoclonal FCM 10149-R103-A
    Anti-Endoglin / CD105 Antibody (PerCP) Mouse Monoclonal FCM 10149-MM13-C
    Anti-Endoglin / CD105 Antibody (APC) Mouse Monoclonal FCM 10149-MM13-A
    Anti-Endoglin / CD105 Antibody Mouse Monoclonal FCM 10149-MM13
    Anti-Endoglin / CD105 Antibody Rabbit Polyclonal WB,ICC/IF,IF 100558-T34
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