Endoglin / CD105 Antibody

Endoglin / CD105 Antibody Datasheet

Endoglin / CD105 Antibody Product Information

• Immunogen: Recombinant Human CD105 protein (Catalog#10149-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 7A6B8A11
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Endoglin / CD105 (rh Endoglin / CD105 ; Catalog#10149-H08H; NP_001108225.1; Met 1-Gly 586). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Endoglin / CD105 Antibody Usage Guide

• Specificity: Human Endoglin / CD105
• No cross-reactivity in ELISA with

  Mouse ENG

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD105 in WB. Using a DAB detection system, the detection limit for Human CD105 is approximately 4 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Endoglin / CD105 Antibody Background

Endoglin, also known as CD105, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type II receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.

References

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