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Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) glycoprotein ORF mammalian expression plasmid, N-His tag

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EBOV EBOV-G cDNA Clone Product Information
NCBI RefSeq:U23187.1
RefSeq ORF Size:2028bp
cDNA Description:Full length Clone DNA of Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) glycoprotein with N terminal His tag.
Gene Synonym:EBOV-G
Species:EBOV
Vector:pCMV3-N-His
Plasmid:pCMV3-His-ZaireEBOV-U23187-GP
Restriction Site:KpnI + XbaI (6kb + 2.08kb)
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
EBOV EBOV-G Gene Plasmid Map
Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) glycoprotein natural ORF mammalian expression plasmid, N-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) glycoprotein ORF mammalian expression plasmid, N-His tag on other vectors
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Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) glycoprotein ORF mammalian expression plasmid, N-Flag tagVG40304-NF$315
Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) glycoprotein ORF mammalian expression plasmid, N-His tagVG40304-NH$315
Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) glycoprotein (Codon Optimized) ORF mammalian expression plasmid, N-Myc tagVG40304-NM$315
Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) glycoprotein ORF mammalian expression plasmid, N-HA tagVG40304-NY$315
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Background

The fourth gene of the EBOV genome encodes a 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD); the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry; the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.

References
  1. Volchkov VE, et al. Processing of the Ebola virus glycoprotein by the proprotein convertase furin. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5762-7.
  2. Lee JE, et al. Structure of the Ebola virus glycoprotein bound to an antibody from a human survivor. Nature. 2008 Jul 10;454(7201):177-82. doi: 10.1038/nature07082.
  3. Hood CL, et al. Biochemical and structural characterization of cathepsin L-processed Ebola virus glycoprotein: implications for viral entry and immunogenicity. J Virol. 2010 Mar;84(6):2972-82. doi: 10.1128/JVI.02151-09.
  4. Cook JD and Lee JE. The secret life of viral entry glycoproteins: moonlighting in immune evasion. PLoS Pathog. 2013 May;9(5):e1003258. doi: 10.1371/journal.ppat.1003258.
  5. Miller EH and Chandran K. Filovirus entry into cells - new insights. Curr Opin Virol. 2012 Apr;2(2):206-14. doi: 10.1016/j.coviro.2012.02.015.
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