Human ESRP1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13708-G	Cloning vector ($195) pCMV / hygro ($445) pCMV / hygro - HA ($445) pCMV / hygro - His ($445) pCMV / hygro - Myc ($445) pCMV / hygro - FLAG ($445)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ESRP1 cDNA Clone Datasheet

ESRP1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_017697.3
cDNA Size:	2046
Gene Synonym:	RBM35A; RMB35A; ESRP1
Source:	Human

ESRP1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens epithelial splicing regulatory protein 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 108 A/T resulting in the amino acid Lys substitution by Asn.

ESRP1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ESRP1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ESRP1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACGGCCT CTCCGGATTA CTTGGTGGTG CTTTTTGGGA TCACTGCTGG GGCCACCGGG
61       GCCAAGCTAG GCTCGGATGA GAAGGAGTTG ATCCTGCTGT TCTGGAATGT CGTGGATCTG
121      GCCAACAAGA AGGTGGGACA GTTGCACGAA GTGCTAGTTA GACCGGATCA GTTGGAACTG
181      ACGGAGGACT GCAAAGAAGA AACTAAAATA GACGTCGAAA GCCTGTCCTC GGCGTCGCAG
241      CTGGACCAAG CCCTCCGACA GTTTAACCAG TCAGTGAGCA ATGAACTGAA TATTGGAGTA
301      GGGACTTCCT TCTGTCTCTG TACTGATGGG CAGCTTCATG TCAGGCAAAT CCTGCATCCT
361      GAGGCTTCCA AGAAGAATGT ACTATTACCT GAATGCTTCT ATTCCTTTTT TGATCTTCGA
421      AAAGAATTCA AGAAATGTTG CCCTGGTTCA CCTGATATTG ACAAACTGGA CGTTGCCACA
481      ATGACAGAGT ATTTAAATTT TGAGAAGAGT AGTTCAGTCT CTCGATATGG AGCCTCTCAA
541      GTTGAAGATA TGGGGAATAT AATTTTAGCA ATGATTTCAG AGCCTTATAA TCACAGGTTT
601      TCAGATCCAG AGAGAGTGAA TTACAAGTTT GAAAGTGGAA CTTGCAGCAA GATGGAACTT
661      ATTGATGATA ACACCGTAGT CAGGGCACGA GGTTTACCAT GGCAGTCTTC AGATCAAGAT
721      ATTGCAAGAT TCTTCAAAGG ACTCAATATT GCCAAGGGAG GTGCAGCACT TTGTCTGAAT
781      GCTCAGGGTC GAAGGAACGG AGAAGCTCTG GTTAGGTTTG TAAGTGAGGA GCACCGAGAC
841      CTAGCACTAC AGAGGCACAA ACATCACATG GGGACCCGGT ATATTGAGGT TTACAAAGCA
901      ACAGGTGAAG ATTTCCTTAA AATTGCTGGT GGTACTTCCA ATGAGGTAGC CCAGTTTCTC
961      TCCAAGGAAA ATCAAGTCAT TGTCCGCATG CGGGGGCTCC CTTTCACGGC CACAGCTGAA
1021     GAAGTGGTGG CCTTCTTTGG ACAGCATTGC CCTATTACTG GGGGAAAGGA AGGCATCCTC
1081     TTTGTCACCT ACCCAGATGG TAGGCCAACA GGGGACGCTT TTGTCCTCTT TGCCTGTGAG
1141     GAATATGCAC AGAATGCGTT GAGGAAGCAT AAAGACTTGT TGGGTAAAAG ATACATTGAA
1201     CTCTTCAGGA GCACAGCAGC TGAAGTTCAG CAGGTGCTGA ATCGATTCTC CTCGGCCCCT
1261     CTCATTCCAC TTCCAACCCC TCCCATTATT CCAGTACTAC CTCAGCAATT TGTGCCCCCT
1321     ACAAATGTTA GAGACTGTAT ACGCCTTCGA GGTCTTCCCT ATGCAGCCAC AATTGAGGAC
1381     ATCCTGGATT TCCTGGGGGA GTTCGCCACA GATATTCGTA CTCATGGGGT TCACATGGTT
1441     TTGAATCACC AGGGCCGCCC ATCAGGAGAT GCCTTTATCC AGATGAAGTC TGCGGACAGA
1501     GCATTTATGG CTGCACAGAA GTGTCATAAA AAAAACATGA AGGACAGATA TGTTGAAGTC
1561     TTTCAGTGTT CAGCTGAGGA GATGAACTTT GTGTTAATGG GGGGCACTTT AAATCGAAAT
1621     GGCTTATCCC CACCGCCATG TAAGTTACCA TGCCTGTCTC CTCCCTCCTA CACATTTCCA
1681     GCTCCTGCTG CAGTTATTCC TACAGAAGCT GCCATTTACC AGCCCTCTGT GATTTTGAAT
1741     CCACGAGCAC TGCAGCCCTC CACAGCGTAC TACCCAGCAG GCACTCAGCT CTTCATGAAT
1801     TACACAGCGT ACTATCCCAG CCCCCCAGGT TCGCCTAATA GTCTTGGCTA CTTCCCTACA
1861     GCTGCTAATC TTAGCGGTGT CCCTCCACAG CCTGGCACGG TGGTCAGAAT GCAGGGCCTG
1921     GCCTACAATA CTGGAGTTAA GGAAATTCTT AACTTCTTCC AAGGTTACCA GTATGCAACC
1981     GAGGATGGAC TTATACACAC AAATGACCAG GCCAGGACTC TACCCAAAGA ATGGGTTTGT
2041     ATTTAA

Quality Control:cDNA sequencing results is available upon request.