Human ESR1 ORF Expression Clone, untagged

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13179-G-N	pCMV / hygro ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ESR1 cDNA Clone Datasheet

ESR1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001122742.1
cDNA Size:	1788
Gene Synonym:	ER; ESR; Era; ESRA; NR3A1; DKFZp686N23123; ESR1
Source:	Human

ESR1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens estrogen receptor 1 DNA.
Vector:	pCMV/hygro
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 30 T/C not causing the amino acid variation.

ESR1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ESR1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ESR1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACCATGA CCCTCCACAC CAAAGCATCC GGGATGGCCC TACTGCATCA GATCCAAGGG
61       AACGAGCTGG AGCCCCTGAA CCGTCCGCAG CTCAAGATCC CCCTGGAGCG GCCCCTGGGC
121      GAGGTGTACC TGGACAGCAG CAAGCCCGCC GTGTACAACT ACCCCGAGGG CGCCGCCTAC
181      GAGTTCAACG CCGCGGCCGC CGCCAACGCG CAGGTCTACG GTCAGACCGG CCTCCCCTAC
241      GGCCCCGGGT CTGAGGCTGC GGCGTTCGGC TCCAACGGCC TGGGGGGTTT CCCCCCACTC
301      AACAGCGTGT CTCCGAGCCC GCTGATGCTA CTGCACCCGC CGCCGCAGCT GTCGCCTTTC
361      CTGCAGCCCC ACGGCCAGCA GGTGCCCTAC TACCTGGAGA ACGAGCCCAG CGGCTACACG
421      GTGCGCGAGG CCGGCCCGCC GGCATTCTAC AGGCCAAATT CAGATAATCG ACGCCAGGGT
481      GGCAGAGAAA GATTGGCCAG TACCAATGAC AAGGGAAGTA TGGCTATGGA ATCTGCCAAG
541      GAGACTCGCT ACTGTGCAGT GTGCAATGAC TATGCTTCAG GCTACCATTA TGGAGTCTGG
601      TCCTGTGAGG GCTGCAAGGC CTTCTTCAAG AGAAGTATTC AAGGACATAA CGACTATATG
661      TGTCCAGCCA CCAACCAGTG CACCATTGAT AAAAACAGGA GGAAGAGCTG CCAGGCCTGC
721      CGGCTCCGCA AATGCTACGA AGTGGGAATG ATGAAAGGTG GGATACGAAA AGACCGAAGA
781      GGAGGGAGAA TGTTGAAACA CAAGCGCCAG AGAGATGATG GGGAGGGCAG GGGTGAAGTG
841      GGGTCTGCTG GAGACATGAG AGCTGCCAAC CTTTGGCCAA GCCCGCTCAT GATCAAACGC
901      TCTAAGAAGA ACAGCCTGGC CTTGTCCCTG ACGGCCGACC AGATGGTCAG TGCCTTGTTG
961      GATGCTGAGC CCCCCATACT CTATTCCGAG TATGATCCTA CCAGACCCTT CAGTGAAGCT
1021     TCGATGATGG GCTTACTGAC CAACCTGGCA GACAGGGAGC TGGTTCACAT GATCAACTGG
1081     GCGAAGAGGG TGCCAGGCTT TGTGGATTTG ACCCTCCATG ATCAGGTCCA CCTTCTAGAA
1141     TGTGCCTGGC TAGAGATCCT GATGATTGGT CTCGTCTGGC GCTCCATGGA GCACCCAGGG
1201     AAGCTACTGT TTGCTCCTAA CTTGCTCTTG GACAGGAACC AGGGAAAATG TGTAGAGGGC
1261     ATGGTGGAGA TCTTCGACAT GCTGCTGGCT ACATCATCTC GGTTCCGCAT GATGAATCTG
1321     CAGGGAGAGG AGTTTGTGTG CCTCAAATCT ATTATTTTGC TTAATTCTGG AGTGTACACA
1381     TTTCTGTCCA GCACCCTGAA GTCTCTGGAA GAGAAGGACC ATATCCACCG AGTCCTGGAC
1441     AAGATCACAG ACACTTTGAT CCACCTGATG GCCAAGGCAG GCCTGACCCT GCAGCAGCAG
1501     CACCAGCGGC TGGCCCAGCT CCTCCTCATC CTCTCCCACA TCAGGCACAT GAGTAACAAA
1561     GGCATGGAGC ATCTGTACAG CATGAAGTGC AAGAACGTGG TGCCCCTCTA TGACCTGCTG
1621     CTGGAGATGC TGGACGCCCA CCGCCTACAT GCGCCCACTA GCCGTGGAGG GGCATCCGTG
1681     GAGGAGACGG ACCAAAGCCA CTTGGCCACT GCGGGCTCTA CTTCATCGCA TTCCTTGCAA
1741     AAGTATTACA TCACGGGGGA GGCAGAGGGT TTCCCTGCCA CGGTCTGA

Quality Control:cDNA sequencing results is available upon request.