Mouse ESM1 / Endocan Protein (His Tag)

Endothelial cell-specific molecule 1 Protein Datasheet

ESM1 / Endocan Protein Product Information

• Synonym: ESM1
• Protein Construction: A DNA sequence encoding the mouse TLR2(Q9QYY7) (Met1-Arg184) was fused with a polyhistidine tag at the C-terminus.
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

ESM1 / Endocan Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 22
• Molecular Mass: The recombinant mouse ESM1 consists of 173 amino acids and has a calculated molecular mass of 19.1 kDa. The recombinant protein migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris,500mM NaCl, pH 8.0, 10% gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ESM1 / Endocan protein

ESM1 / Endocan Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ESM1 / Endocan Protein Description

ESM1 is a secreted protein which is produced by adipocytes. It is mainly expressed in the endothelial cells in human lung and kidney tissues. It has been noticed that ESM1 may play some role in obesity-associated vascular disease since circulating ESM-1 levels are reduced in the overweight and obese. It has been described as a specific biomarker of tip cells during neoangiogenesis. The expression of ESM1 gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF or FGF-2. In hypervascularized cancers, overexpression of endocan has been detected by immunohistochemistry using monoclonal antibodies against ESM1.

References

1. Cong R. et al., 2006, Biophys Res Commun. 346 (2): 535-45.
2. Aitkenhead M. et al., 2002, Microvasc Res. 63 (2): 159-71.
3. Lassalle P. et al., 1996, J Biol Chem. 271 (34): 20458-64.