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Rat ESAM Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| RG80241-G |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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ESAM cDNA Clone Datasheet
ESAM cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_001004245.1 |
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cDNA Size: |
1185 |
| Gene Synonym: |
MGC94065; Esam |
| Source: | Rat |
ESAM cDNA Clone Description
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cDNA Description: |
ORF Clone of Rattus norvegicus endothelial cell adhesion molecule DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref. ID sequence except for the point mutation 33 C/G resulting in the amino acid Ser substitution by Arg. |
ESAM cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
ESAM cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
ESAM cDNA Clone Sequence and Quality Control
Sequence:
1 ATGATTCTTC CGGCCAGAAC CCCCGAGACC AGGTTACTGC GGGTTTTGTT CCTGGGACTG 61 AGTACCCTTG CTGCCTTCTC ACTGGCTCAG ATGGAGCTGC ACGTGCCCCC TGGCCTCAAC 121 AAATTGGAGG CGGTAGAGGG GGAAGAAGTG GTGCTCCCCG CCTGGTACAC GATGGCACGG 181 GAGGAGTCTT CATCCCACCC CTGGGAGGCG CCCTTCCTGA TTTGGTTCTT GGAACAAGAA 241 GGGAAGGAAC CAAAGCAGGT CTTGTCTTAT TTCAAGGGTT CCCTGTCAAA TAAACCTGGA 301 GTAACCCTGG TCCACTCCAT CTCCACACGG AATGTGTCCC TGCGCCTGGA TGCCCTCCAG 361 GAGGGAGACT CTGGGACCTA CCGCTGTTCT GTCAATGCGT ACGACAGTGA CGGCAAAAAT 421 ATAGGCCACA GCATCAAAAC GATAGAGCTC AAAGTGCTGG TTCCTCCAGC TCCTCCATCC 481 TGTAGTTTTC AGGGTGTACC CTATGTGGGG ACCAATGTGA CTCTGAACTG CAAGTCCCCA 541 AGGAGTAAAC CTACTGCTCA ATACCAGTGG GAGAGGCTGG CGCCATCCTC CCAGGTCTTC 601 TTTGGACCAG CCTTAGATAC CGTCCGTGGA TCTTTGAAGC TCACCAACAT TTCCACTGCC 661 ATGTCTGGAG TCTATGTCTG CAAGGCCCAA AACAGAGTGG GCTTTGCCCA GTGCAATGTG 721 ACCTTGGACG TGATGACAGG GTCCAAGGCT GCTGTGGTCG CTGGAGCAGT GGTGGGCACT 781 TTTGTTGGGT TGGTGCTGAT AGCTGGTCTG GTCCTGTTGT ACCAACGCCG CAGCAAGACC 841 CTGGAAGAGC TGGCCAACGA TATCAAGGAA GATGCCATTG CTCCCCGGAC CTTGCCTTGG 901 ACCAAAGGCT CAGACACAAT CTCCAAGAAT GGGACACTTT CCTCAGTCAC CTCAGCACGA 961 GCTCTGCGGC CACCCAAAGC TGCTCCTCCA AGACCTGGCA CGTTTACTCC TACACCCAGT 1021 GTCTCTAGCC AGGCCCTGTC CTCACCCAGA CTGCCTAGGA CGGATGGGCC GCCACCTCAG 1081 GCAGTGTCCC TGACCCCAGG TGGGGTTTCT TCTTCTACTC TGAACCGCAT GGGTGCCGTG 1141 CCCGTGATGG TACCTGCACA GAGTCAGGCC GGGTCTCTCG TGTGA
Quality Control:cDNA sequencing results is available upon request.
